Rozman Nur Amiera Syuhada Binti, Hamin Nurhanis Syafiqah Binti Mohd Nor, Ring Leong Chean, Nee Tan Wen, Mustapha Mahfuzah Binti, Yenn Tong Woei
Universiti Kuala Lumpur, Malaysian Institute of Chemical and Bioengineering Technology, 78000 Alor Gajah, Melaka, Malaysia.
School of Distance Education, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
Mycobiology. 2017 Sep;45(3):178-183. doi: 10.5941/MYCO.2017.45.3.178. Epub 2017 Sep 30.
Diabetes mellitus is a chronic disorder which affects millions of population worldwide. Global estimates published in 2010 reported the world diabetic prevalence as 6.4%, affecting 285 million adults. Foot ulceration and wound infection are major forms of disabilities arising from diabetic diseases. This study was aimed to develop a natural antimicrobial finishing on medical grade textile that meets American Association of Textiles Chemists and Colorists (AATCC) standard. The textile samples were finished with the ethanolic extract of elv609, an endophytic fungus isolated from Benth (common name: cat's whiskers). Endophyte is defined as microorganism that reside in the living plant tissue, without causing apparent disease symptom to the host. The antimicrobial efficacy of the ethanolic extract of was tested on clinical pathogens isolated from diabetic wound. The extract exhibited significant inhibitory activity against 4 bacteria and 1 yeast with the minimal inhibitory concentration ranged from 6.25 to 12.5 mg/mL. The results indicate different susceptibility levels of the test microorganism to the ethanolic extract. However, the killing activity of the extract was concentration-dependent. The finished medical textile showed excellent antimicrobial efficacy on AATCC test assays. All the microbial cultures treated with the textile sample displayed a growth reduction of 99.9% on Hoheinstein Challenge Test. The wash durability of the finished textile was found good even after 50 washes with commercial detergent. Besides, the gas chromatography mass spectrometry analysis showed that 6-octadecenoic acid and diethyl phthalate were the main bioactive constituents of the extract. In conclusion, the developed medical textile showed good antimicrobial efficacy on laboratory tests. This work can be extended to trials for developing healthcare textile products for antimicrobial applications.
糖尿病是一种慢性疾病，影响着全球数百万人。2010年发布的全球估计报告称，全球糖尿病患病率为6.4%，影响了2.85亿成年人。足部溃疡和伤口感染是糖尿病相关疾病导致的主要残疾形式。本研究旨在开发一种符合美国纺织化学家和染色家协会（AATCC）标准的医用级纺织品天然抗菌整理剂。纺织品样品用elv609的乙醇提取物进行整理，elv609是一种从Benth（俗名：猫须草）中分离出的内生真菌。内生菌被定义为存在于活植物组织中，且不会对宿主造成明显疾病症状的微生物。该乙醇提取物对从糖尿病伤口分离出的临床病原体的抗菌效果进行了测试。该提取物对4种细菌和1种酵母菌表现出显著的抑制活性，最低抑菌浓度范围为6.25至12.5mg/mL。结果表明测试微生物对乙醇提取物的敏感性水平不同。然而，提取物的杀菌活性呈浓度依赖性。整理后的医用纺织品在AATCC测试中显示出优异的抗菌效果。用纺织品样品处理的所有微生物培养物在霍恩斯坦挑战试验中生长减少了99.9%。即使在用商业洗涤剂洗涤50次后，整理后纺织品的耐洗性仍良好。此外，气相色谱-质谱分析表明，6-十八碳烯酸和邻苯二甲酸二乙酯是该提取物的主要生物活性成分。总之，开发的医用纺织品在实验室测试中显示出良好的抗菌效果。这项工作可以扩展到临床试验，以开发用于抗菌应用的医疗保健纺织品产品。
