Lawman Samuel, Madden Peter W, Romano Vito, Dong Yue, Mason Sharon, Williams Bryan M, Kaye Stephen B, Willoughby Colin E, Harding Simon P, Shen Yao-Chun, Zheng Yalin
Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, L69 3GJ, UK.
Department of Eye and Vision Science, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, L7 8TX, UK.
Biomed Opt Express. 2017 Nov 13;8(12):5579-5593. doi: 10.1364/BOE.8.005579. eCollection 2017 Dec 1.
Optical coherence tomography (OCT) can monitor human donor corneas non-invasively during the de-swelling process following storage for corneal transplantation, but currently only resultant thickness as a function of time is extracted. To visualize and quantify the mechanism of de-swelling, we present a method exploiting the nanometer sensitivity of the Fourier phase in OCT data to image deformation velocities. The technique was demonstrated by non-invasively showing during de-swelling that osmotic flow through an intact epithelium is negligible and removing the endothelium approximately doubled the initial flow at that interface. The increased functional data further enabled the validation of a mathematical model of the cornea. Included is an efficient method of measuring high temporal resolution (1 minute demonstrated) corneal thickness, using automated collection and semi-automated graph search segmentation. These methods expand OCT capabilities to measure volume change processes for tissues and materials.
光学相干断层扫描（OCT）能够在供体角膜储存后用于角膜移植的消肿过程中对其进行无创监测，但目前仅提取了作为时间函数的最终厚度。为了可视化和量化消肿机制，我们提出了一种利用OCT数据中傅里叶相位的纳米级灵敏度来成像变形速度的方法。通过在消肿过程中无创显示完整上皮细胞的渗透流可忽略不计，以及去除内皮细胞后该界面处的初始流量增加了约一倍，证明了该技术的有效性。增加的功能数据进一步验证了角膜的数学模型。其中包括一种测量高时间分辨率（已证明为1分钟）角膜厚度的有效方法，该方法采用自动采集和半自动图形搜索分割。这些方法扩展了OCT测量组织和材料体积变化过程的能力。
