Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Research Institute of Horticulture, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Int J Mol Sci. 2018 Jan 2;19(1):118. doi: 10.3390/ijms19010118.
is a major tree species for producing edible oil. Its seed oil is well known for the high level of oleic acids; however, little is known regarding the molecular mechanism of lipid biosynthesis in . Here, we measured the oil contents and fatty acid (FA) compositions at four developmental stages and investigated the global gene expression profiles through transcriptomics sequencing. We identified differentially-expressed genes (DEGs) among the developmental stages and found that the distribution of numbers of DEGs was associated with the accumulation pattern of seed oil. Gene Ontology (GO) enrichment analysis revealed some critical biological processes related to oil accumulation, including lipid metabolism and phosphatidylcholine metabolism. Furthermore, we investigated the expression patterns of lipid biosynthesis genes. We showed that most of the genes were identified with single or multiple copies, and some had correlated profiles along oil accumulation. We proposed that the higher levels of () coupled with lower activities of () might be responsive to the boost of oleic acid at the late stage of seeds' development. This work presents a comprehensive transcriptomics study of seeds and uncovers valuable DEGs that are associated with the seed oil accumulation.
是一种主要的产食用油树种。其种子油以高油酸含量而闻名；然而，关于 中脂质生物合成的分子机制知之甚少。在这里，我们在四个发育阶段测量了油含量和脂肪酸 (FA) 组成，并通过转录组测序研究了全局基因表达谱。我们在发育阶段之间鉴定了差异表达基因 (DEG)，并发现 DEG 的数量分布与种子油的积累模式有关。基因本体论 (GO) 富集分析揭示了一些与油脂积累相关的关键生物学过程，包括脂质代谢和磷脂酰胆碱代谢。此外，我们还研究了脂质生物合成基因的表达模式。结果表明，大多数基因都具有单个或多个拷贝，并且一些基因与油脂积累具有相关的表达模式。我们提出，较高水平的 () 加上 () 活性较低，可能是对种子发育后期油酸水平升高的响应。这项工作对 种子进行了全面的转录组学研究，揭示了与种子油积累相关的有价值的差异表达基因。
