Choi Kang Duk, Lee Ga-Eun, Park Jin-Sook
Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 456-749, Republic of Korea.
Sellusone Co. Ltd, 327 Chungang-no Anseong-si, Kyonggi-do 456-749, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Apr;68(4):990-994. doi: 10.1099/ijsem.0.002575. Epub 2018 Feb 14.
A Gram-reaction-negative, aerobic, motile by gliding, non-spore-forming, rod-shaped bacterial strain, designated 122CH820-2, was isolated from spongin. This bacterium was characterized to determine its taxonomic position by using a polyphasic approach. Strain 122CH820-2 grew well at 25-30 °C on marine agar. On the basis of 16S rRNA gene sequence similarity, strain 122CH820-2 belonged to the family Flavobacteriaceae and was closely related to Aquimarina mytili PSC33 (96.8 % sequence similarity) and A. penaei P3-1 (96.7 %). Lower sequence similarities (<96.5 %) were found with all of the other recognized members of the genus Aquimarina. The G+C content of the genomic DNA was 35.2 mol%. The major respiratory quinone was menaquinone MK-6 and the major fatty acids were C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. The polar lipids were phosphatidylethanolamine, one aminophospholipid and five unidentified polar lipids. Strain 122CH820-2 could be differentiated genotypically and phenotypically from recognized species of the genus Aquimarina. The isolate therefore represents a novel species, for which the name Aquimarina spongiicola sp. nov. is proposed, with the type strain 122CH820-2 (=KACC 19274=LMG 30078).
从海绵体中分离出一株革兰氏阴性、需氧、滑行运动、不形成芽孢的杆状细菌菌株，命名为122CH820-2。采用多相分类法对该细菌进行特征分析以确定其分类地位。菌株122CH820-2在25-30°C的海洋琼脂培养基上生长良好。基于16S rRNA基因序列相似性，菌株122CH820-2属于黄杆菌科，与贻贝海栖菌PSC33（序列相似性为96.8%）和对虾海栖菌P3-1（96.7%）密切相关。与海栖菌属其他所有已确认成员的序列相似性较低（<96.5%）。基因组DNA的G+C含量为35.2 mol%。主要呼吸醌为甲基萘醌MK-6，主要脂肪酸为C15 : 0、异-C17 : 0 3-OH、异-C15 : 1 G和异-C15 : 0 3-OH。极性脂质为磷脂酰乙醇胺、一种氨基磷脂和五种未鉴定的极性脂质。菌株122CH820-2在基因型和表型上可与海栖菌属已确认的物种区分开来。因此，该分离株代表一个新物种，提议将其命名为海绵海栖菌（Aquimarina spongiicola sp. nov.），模式菌株为122CH820-2（=KACC 19274=LMG 30078）。
