Chui Chih-Yao, Mouthuy Pierre-Alexis, Ye Hua
Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Old Road Campus, Oxford, OX3 7DQ, UK.
Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Windmill Road, Oxford, OX3 7LD, UK.
Biotechnol Lett. 2018 Apr;40(4):737-744. doi: 10.1007/s10529-018-2522-7. Epub 2018 Feb 15.
To demonstrate that uniform poly(vinyl butyral) (PVB) fibres can be safely electrospun onto a monolayer of human dermal fibroblasts using a portable device.
PVB in solvent mixtures containing various amounts of ethanol and water was electrospun. Six percent (weight-to-volume ratio) PVB in a 9:1 ethanol:water ratio was the solution with the highest content in water that could be electrospun into consistent fibres with an average diameter of 0.9 μm (± 0.1 μm). Four and five percent PVB solutions created beaded fibres. A 8:2 ethanol:water solution lead to microbead formation while a 7:3 ethanol:water mix failed to fully dissolve. The selected solution was successfully electrospun onto a monolayer of human dermal fibroblasts and the process had no significant effect (p < 0.05) on cell viability compared to the control without fibres.
PVB-ethanol-water solutions could be electrospun without damaging the exposed cell layer. However, further work is required to demonstrate the long-term effect of PVB as a wound healing material.
证明使用便携式设备可将均匀的聚乙烯醇缩丁醛（PVB）纤维安全地静电纺丝到人类真皮成纤维细胞单层上。
对含有不同乙醇和水含量的溶剂混合物中的PVB进行静电纺丝。在9:1乙醇：水比例的溶剂混合物中，6%（重量/体积比）的PVB是水含量最高且能静电纺丝成平均直径为0.9μm（±0.1μm）的均匀纤维的溶液。4%和5%的PVB溶液形成了串珠状纤维。8:2乙醇：水溶液导致微珠形成，而7:3乙醇：水混合物未能完全溶解。所选溶液成功地静电纺丝到人类真皮成纤维细胞单层上，与没有纤维的对照组相比，该过程对细胞活力没有显著影响（p < 0.05）。
PVB-乙醇-水溶液可以进行静电纺丝而不损伤暴露的细胞层。然而，需要进一步的研究来证明PVB作为伤口愈合材料的长期效果。
