The John B. Pierce Laboratory, Inc., New Haven, CT 06519, United States; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, United States.
The John B. Pierce Laboratory, Inc., New Haven, CT 06519, United States; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, United States; Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06510, United States.
Curr Opin Neurobiol. 2018 Jun;50:146-153. doi: 10.1016/j.conb.2018.02.006. Epub 2018 Mar 20.
In order to understand how brain activity produces adaptive behavior we need large-scale, high-resolution recordings of neuronal activity. Fluorescent genetically encoded voltage indicators (GEVIs) offer the potential for these recordings to be performed chronically from targeted cells in a minimally invasive manner. As the number of GEVIs successfully tested for in vivo use grows, so has the number of open questions regarding the improvements that would facilitate broad adoption of this technology that surpasses mere 'proof of principle' studies. Our aim in this review is not to provide a status check of the current state of the field, as excellent publications covering this topic already exist. Here, we discuss specific questions regarding GEVI development and application that we think are crucial in achieving this goal.
为了理解大脑活动如何产生适应性行为，我们需要对神经元活动进行大规模、高分辨率的记录。荧光遗传编码电压指示剂 (GEVI) 提供了以微创方式从靶向细胞进行长期记录的潜力。随着成功用于体内检测的 GEVIs 的数量增加，关于促进这项技术广泛应用的问题也越来越多，这些问题超越了仅仅“原理验证”研究。我们在这篇综述中的目的不是提供该领域当前状况的最新情况，因为已经有优秀的出版物涵盖了这个主题。在这里，我们讨论了关于 GEVI 开发和应用的具体问题，我们认为这些问题对于实现这一目标至关重要。
