Silvan Jose M, Zorraquin-Peña Irene, Gonzalez de Llano Dolores, Moreno-Arribas M Victoria, Martinez-Rodriguez Adolfo J
Grupo de Microbiología y Biocatálisis de Alimentos, Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de la Alimentación, CSIC-UAM, Madrid, Spain.
Grupo de Biotecnología Enológica Aplicada, Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de la Alimentación, CSIC-UAM, Madrid, Spain.
Front Microbiol. 2018 Mar 16;9:458. doi: 10.3389/fmicb.2018.00458. eCollection 2018.
is the leading cause of bacterial diarrheal disease worldwide. Although most episodes of campylobacteriosis are self-limiting, antibiotic treatment is usually needed in patients with serious enteritis, and especially in childrens or the elderly. In the last years, antibiotic resistance in has become a major public health concern and a great interest exists in developing new antimicrobial strategies for reducing the impact of this food-borne pathogen on human health. Among them, the use of silver nanoparticles as antibacterial agents has taken on increased importance in the field of medicine. The aim of the present study was to evaluate the antimicrobial effectiveness of glutathione-stabilized silver nanoparticles (GSH-Ag NPs) against multidrug resistant (MDR) strains isolated from the chicken food chain (FC) and clinical patients (C). The results obtained showed that GSH-Ag NPs were highly effective against all MDR strains tested. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were in a range from 4.92 to 39.4 μg/mL and 9.85 to 39.4 μg/mL, respectively. Cytotoxicity assays were also assessed using human intestinal HT-29, Caco-2, and CCD-18 epithelial cells. Exposure of GSH-Ag NPs to intestinal cells showed a dose-dependent cytotoxic effect in all cell lines between 9.85 and 39.4 μg/mL. More than 60% of the tested strains were susceptible to GSH-Ag NPs concentrations ≤ 9.85 μg/mL, suggesting that practical inhibitory levels could be reached at low GSH-Ag NPs concentrations. Further works are needed with the purpose to evaluate the practical implications of the toxicity studies and to know more about other attributes linked to the biological compatibility. This behavior makes GSH-Ag NPs as a promising tool for the design of novel antibacterial agents for controlling .
是全球细菌性腹泻疾病的主要病因。虽然大多数弯曲杆菌病发作是自限性的，但重症肠炎患者通常需要抗生素治疗，尤其是儿童或老年人。在过去几年中，弯曲杆菌的抗生素耐药性已成为一个主要的公共卫生问题，人们对开发新的抗菌策略以减少这种食源性病原体对人类健康的影响非常感兴趣。其中，使用银纳米颗粒作为抗菌剂在医学领域变得越来越重要。本研究的目的是评估谷胱甘肽稳定的银纳米颗粒（GSH-Ag NPs）对从鸡肉食物链（FC）和临床患者（C）分离出的多重耐药（MDR）弯曲杆菌菌株的抗菌效果。获得的结果表明，GSH-Ag NPs对所有测试的MDR弯曲杆菌菌株都非常有效。最小抑菌浓度（MIC）和最小杀菌浓度（MBC）分别在4.92至39.4μg/mL和9.85至39.4μg/mL范围内。还使用人肠道HT-29、Caco-2和CCD-18上皮细胞评估了细胞毒性试验。GSH-Ag NPs暴露于肠道细胞显示在9.85至39.4μg/mL的所有细胞系中都有剂量依赖性细胞毒性作用。超过60%的测试弯曲杆菌菌株对≤9.85μg/mL的GSH-Ag NPs浓度敏感，这表明在低GSH-Ag NPs浓度下可以达到实际抑制水平。需要进一步开展工作，以评估毒性研究的实际意义，并更多地了解与生物相容性相关的其他特性。这种特性使GSH-Ag NPs成为设计用于控制弯曲杆菌的新型抗菌剂的有前途的工具。
