Department of Dental Hygiene, Kyungdong University, Wonju 26495, South Korea.
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 31116, South Korea.
Sci Rep. 2018 Apr 4;8(1):5663. doi: 10.1038/s41598-018-23939-6.
Restoration of hard tissue in conjunction with adhesive is a globally challenging issue in medicine and dentistry. Common clinical therapies involving application of adhesive and substitute material for functional or anatomical recovery are still suboptimal. Biomaterials with bioactivity and inhibitory effects of enzyme-mediated adhesive degradation can render a solution to this. Here, we designed a novel copper-doped bioactive glass nanoparticles (CuBGn) to offer multifunction: metalloproteinases (MMP) deactivation and remineralization and incorporated the CuBGn in resin-dentin adhesive systems, which showed most common failure of MMP mediated adhesive degradation among hard tissue adhesives, to evaluate proposed therapeutic effects. A sol-gel derived bioactive glass nanoparticles doping 10 wt% of Cu (Cu-BGn) for releasing Cu ions, which were well-known MMP deactivator, were successfully created and included in light-curing dental adhesive (DA), a filler-free co-monomer resin blend, at different concentrations (up to 2 wt%). These therapeutic adhesives (CuBGn-DA) showed enhanced (a)cellular bioactivity, cytocompatibility, microtensile bond strength and MMP deactivation-ability. In conclusion, the incorporation of Cu ions releasing nano-bioactive glass demonstrated multifunctional properties at the resin-dentin interface; MMP deactivation and remineralization, representing a suitable strategy to extend the longevity of adhesive-hard tissue (i.e. resin-dentin) interfaces.
在医学和牙科领域，结合黏附剂修复硬组织是一个全球性的挑战。常见的临床治疗方法包括应用黏附剂和替代材料来恢复功能或解剖结构，但效果仍不理想。具有生物活性和抑制酶介导黏附剂降解作用的生物材料可以为此提供解决方案。在这里，我们设计了一种新型的铜掺杂生物活性玻璃纳米颗粒（CuBGn），以提供多种功能：金属蛋白酶（MMP）失活和再矿化，并将 CuBGn 掺入树脂-牙本质黏附系统中，该系统显示了最常见的硬组织黏附剂中 MMP 介导的黏附剂降解失效，以评估拟议的治疗效果。成功制备了一种溶胶-凝胶衍生的生物活性玻璃纳米颗粒，掺杂了 10wt%的 Cu（Cu-BGn）以释放 Cu 离子，Cu 离子是众所周知的 MMP 失活剂，并将其包含在光固化牙科黏合剂（DA）中，这是一种无填料的共聚单体树脂混合物，浓度不同（高达 2wt%）。这些治疗性黏合剂（CuBGn-DA）表现出增强的（a）细胞生物活性、细胞相容性、微拉伸黏结强度和 MMP 失活能力。总之，在树脂-牙本质界面处掺入释放纳米生物活性玻璃的 Cu 离子表现出多功能特性；MMP 失活和再矿化，代表了延长黏附-硬组织（即树脂-牙本质）界面寿命的合适策略。
