Faculty of Engineering and Agricultural Sciences, Pontifical Catholic University of Argentina, Buenos Aires C1107AAZ, Argentina.
National Scientific and Technical Research Council (CONICET), Buenos Aires C1425FQB, Argentina.
Molecules. 2018 Apr 7;23(4):842. doi: 10.3390/molecules23040842.
Red wine polyphenols are known for their implications for human health protection, although they suffer from high instability. For this reason, a red wine powder was prepared by freeze-drying encapsulation in maltodextrin/arabic gum matrix, and its composition was determined by means of high-performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (HPLC-MS-QTOF). More than thirty polyphenols, including anthocyanins, flavanols, flavonols, phenolic acids and stilbenoids, were identified. Some of the main quantified polyphenols were: malvidin-3--glucoside, malvidin 3--(6″-acetyl-glucose), petunidin-3--glucoside, quercetin-3--glucuronide, syringenin-3--glucoside, epicatechin, gallic acid and syringic acid. The biological activity of this de-alcoholized and encapsulated red wine on human neuroblastoma SH-SY5Y cells was studied. The results showed that the encapsulated red wine powder has active redox properties, as verified by performing reactive oxygen species (ROS) analysis utilizing a neuronal model. This could help explain its action against the neurotoxicity induced by 6-hydroxydopamine (6-OHDA).
红酒多酚因其对人类健康保护的影响而闻名，尽管它们的稳定性较差。出于这个原因，通过在麦芽糊精/阿拉伯胶基质中进行冷冻干燥包封，制备了红酒粉，并通过高效液相色谱-四极杆飞行时间质谱联用（HPLC-MS-QTOF）来确定其组成。鉴定出了三十多种多酚，包括花青素、黄烷醇、黄酮醇、酚酸和芪类。一些主要定量的多酚是：矢车菊素-3--葡萄糖苷、矢车菊素 3--（6″-乙酰-葡萄糖）、芍药素-3--葡萄糖苷、槲皮素-3--葡萄糖醛酸苷、丁香苷-3--葡萄糖苷、表儿茶素、没食子酸和丁香酸。研究了这种去醇化和封装的红酒对人神经母细胞瘤 SH-SY5Y 细胞的生物学活性。结果表明，封装的红酒粉具有活性的氧化还原性质，这通过利用神经元模型进行活性氧（ROS）分析得到了验证。这可以帮助解释其对 6-羟基多巴胺（6-OHDA）诱导的神经毒性的作用。
