Ren Qian, Li Zhongcheng, Ding Longjiang, Wang Xiuqing, Niu Yumei, Qin Xi, Zhou Xuedong, Zhang Linglin
State Key Laboratory of Oral Diseases; National Clinical Research Center for Oral Diseases; and Department of Cariology and Endodonics, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section, Renmin Nan Lu, Chengdu, People's Republic of China.
Regen Biomater. 2018 Mar;5(2):69-76. doi: 10.1093/rb/rby005. Epub 2018 Mar 21.
In this study, we have designed a more clinically powerful anti-caries treatment by applying the amelogenin-derived peptide QP5 to the antibacterial carrier material chitosan in a hydrogel (CS-QP5 hydrogel), and characterized its effects on the inhibition of a cariogenic biofilm and the promotion of the remineralization of the initial caries lesions. The results indicated that the CS-QP5 hydrogel sustainably inhibited the growth of the biofilm, lactic acid production and the metabolic activity over a prolonged period of time. Moreover, the CS-QP5 hydrogel promoted the remineralization of early enamel lesions, which were indicated by surface micro-hardness (, polarized light microscopy and transverse microradiography. In conclusion, the CS-QP5 hydrogel shows good potential for caries control in the clinic because of its antibacterial effects as well as the remineralization of initial enamel carious lesions even in a biofilm model over a prolonged period of time.
在本研究中，我们通过将牙釉蛋白衍生肽QP5应用于水凝胶中的抗菌载体材料壳聚糖（CS-QP5水凝胶），设计了一种临床效果更强的抗龋治疗方法，并对其抑制致龋生物膜和促进早期龋损再矿化的效果进行了表征。结果表明，CS-QP5水凝胶在较长时间内可持续抑制生物膜生长、乳酸产生和代谢活性。此外，CS-QP5水凝胶促进了早期牙釉质病变的再矿化，这通过表面显微硬度、偏振光显微镜和横向显微放射摄影得以证实。总之，CS-QP5水凝胶因其抗菌作用以及即使在生物膜模型中长时间对早期牙釉质龋损的再矿化作用，在临床上显示出良好的龋齿控制潜力。
