Kunder Mamatha, Lakshmaiah V, Moideen Kutty A V
1Department of Biochemistry, Sri Devaraj Urs Medical College, A Constituent College of Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar, Karnataka 563103 India.
2Department of Medicine, Sri Devaraj Urs Medical College, A Constituent College of Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar, Karnataka India.
Indian J Clin Biochem. 2018 Apr;33(2):218-221. doi: 10.1007/s12291-017-0658-1. Epub 2017 May 2.
Dengue fever (DF) is characterized by systemic inflammatory response including neutrophil activation leading to uncontrolled elastase activity. This study was aimed to measure the activity of plasma neutrophil elastase (NE), its endogenous inhibitors α-antitrypsin (α-AT) and α-macroglobulin (α-MG) and elastase in complex with α-AT (NE-α-AT complex) in DF. 50 dengue patients [39 DF and 11 dengue hemorrhagic fever (DHF)] and 52 healthy subjects were included in the study. NE was measured using -succinyl-tri-alanine--nitroanilide as substrate. α-AT, α-MG and NE-α-AT complex were estimated by ELISA. The result analysis indicated that the dengue patients had significantly higher elastase activity with significantly reduced inhibitor levels compared to controls. Between DF and DHF patients, DHF group had significantly higher elastase activity. In conclusion, significantly elevated NE and reduced inhibitors level in dengue fever indicate these parameters could be of significance in DF particularly for the assessment of progression of inflammatory processes.
登革热（DF）的特征是全身炎症反应，包括中性粒细胞活化导致弹性蛋白酶活性失控。本研究旨在测量登革热患者血浆中性粒细胞弹性蛋白酶（NE）、其内源性抑制剂α-抗胰蛋白酶（α-AT）和α-巨球蛋白（α-MG）以及与α-AT结合的弹性蛋白酶（NE-α-AT复合物）的活性。该研究纳入了50名登革热患者[39例登革热（DF）和11例登革出血热（DHF）]以及52名健康受试者。以-琥珀酰-三丙氨酸--硝基苯胺为底物测量NE。通过酶联免疫吸附测定法（ELISA）评估α-AT、α-MG和NE-α-AT复合物。结果分析表明，与对照组相比，登革热患者的弹性蛋白酶活性显著更高，而抑制剂水平显著降低。在DF和DHF患者之间，DHF组的弹性蛋白酶活性显著更高。总之，登革热中NE显著升高和抑制剂水平降低表明这些参数在登革热中可能具有重要意义，特别是对于评估炎症过程的进展。
