Kook Sun-Young, Kim Yunjeong, Kang Ben, Choe Yon Ho, Kim Young-Ho, Kim Seokjin
Probioticslab R&D Institute, Bioeleven Co., Seoul, Korea.
Department of Pediatric, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Intest Res. 2018 Apr;16(2):246-254. doi: 10.5217/ir.2018.16.2.246. Epub 2018 Apr 30.
BACKGROUND/AIMS: Tens of trillions of microorganisms constitute the gut microbiota of the human body. The microbiota plays a critical role in maintaining host immunity and metabolism. Analyses of the gut microbial composition in Korea are limited to a few studies consisting of small sample sizes. To investigate the gut microbial community in a large sample of healthy Koreans, we analyzed the 16S ribosomal RNA of 4 representative bacterial genera , , , and .
A total of 378 DNA samples extracted from 164 infants and 214 adults were analyzed using quantitative real-time polymerase chain reaction.
Analysis of 16S ribosomal RNA of 4 representative bacterial genera , , , and showed that the gut microbiota in infants had higher relative abundances of and than that in adults, which was dominated by and .
To the best of our knowledge, this was the first study evaluating the distinct characteristics of the microbial community of Korean infants and adults. The differences between the 2 populations suggest that external factors such as age, diet, and the environment are important contributing factors to the change in gut microbial composition during development.
背景/目的：数万亿微生物构成了人体肠道微生物群。微生物群在维持宿主免疫和代谢方面发挥着关键作用。韩国对肠道微生物组成的分析仅限于少数样本量较小的研究。为了调查大量健康韩国人的肠道微生物群落，我们分析了4个代表性细菌属（ 、 、 、 ）的16S核糖体RNA。
使用定量实时聚合酶链反应分析了从164名婴儿和214名成年人中提取的378个DNA样本。
对4个代表性细菌属（ 、 、 、 ）的16S核糖体RNA分析表明，婴儿肠道微生物群中 和 的相对丰度高于成年人，成年人肠道微生物群以 和 为主。
据我们所知，这是第一项评估韩国婴儿和成年人微生物群落不同特征的研究。这两个人群之间的差异表明，年龄、饮食和环境等外部因素是发育过程中肠道微生物组成变化的重要促成因素。
