Ernst J F, Chan R K
J Bacteriol. 1985 Jul;163(1):8-14. doi: 10.1128/jb.163.1.8-14.1985.
We describe mutants of Saccharomyces cerevisiae that are more sensitive than the wild type to the aminoglycoside antibiotics G418, hygromycin B, destomycin A, and gentamicin X2. In addition, the mutants are sensitive to apramycin, kanamycin B, lividomycin A, neamine, neomycin, paromomycin, and tobramycin--antibiotics which do not inhibit wild-type strains. Mapping studies suggest that supersensitivity is caused by mutations in at least three genes, denoted AGS1, AGS2, and AGS3 (for aminoglycoside antibiotic sensitivity). Mutations in all three genes are required for highest antibiotic sensitivity; ags1 ags2 double mutants have intermediate antibiotic sensitivity. AGS1 was mapped 8 centimorgans distal from LEU2 on chromosome III. Analyses of yeast strains transformed with vectors carrying antibiotic resistance genes revealed that G418, gentamicin X2, kanamycin B, lividomycin A, neamine, and paromomycin are inactivated by the Tn903 phosphotransferase and that destomycin A is inactivated by the hygromycin B phosphotransferase. ags strains are improved host strains for vectors carrying the phosphotransferase genes because a wide spectrum of aminoglycoside antibiotics can be used to select for plasmid maintenance.
我们描述了酿酒酵母的突变体，它们对氨基糖苷类抗生素G418、潮霉素B、龟裂链霉素A和庆大霉素X2比野生型更敏感。此外，这些突变体对阿泊拉霉素、卡那霉素B、青紫霉素A、新霉素、新霉素、巴龙霉素和妥布霉素敏感，而这些抗生素不抑制野生型菌株。定位研究表明，超敏性是由至少三个基因的突变引起的，分别命名为AGS1、AGS2和AGS3（氨基糖苷类抗生素敏感性）。所有三个基因的突变对于最高的抗生素敏感性是必需的；ags1 ags2双突变体具有中等的抗生素敏感性。AGS1定位在第三条染色体上距LEU2 8厘摩处。对用携带抗生素抗性基因的载体转化的酵母菌株的分析表明，G418、庆大霉素X2、卡那霉素B、青紫霉素A、新霉素和巴龙霉素被Tn903磷酸转移酶灭活，而龟裂链霉素A被潮霉素B磷酸转移酶灭活。ags菌株是携带磷酸转移酶基因载体的优良宿主菌株，因为可以使用多种氨基糖苷类抗生素来选择质粒的维持。
