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在粪便和细胞培养物中观察到的膜相关病毒复合物。

Membrane-associated viral complexes observed in stools and cell culture.

作者信息

Williams F P

出版信息

Appl Environ Microbiol. 1985 Aug;50(2):523-6. doi: 10.1128/aem.50.2.523-526.1985.

DOI:10.1128/aem.50.2.523-526.1985
PMID:2996424
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238652/
Abstract

Viral complexes observed to be membrane associated rather than clumped by antibody were detected in a rotavirus-containing stool specimen by negative-stain electron microscopy. These "viral packets" were also observed in cell culture fluids after repeated passaging and contained up to 100 virions. Other stool specimens have been observed to contain similar packets of parvovirus-like particles. Such complexes must be expected in fecally contaminated water.

摘要

通过负染色电子显微镜在一份含有轮状病毒的粪便标本中检测到病毒复合体,这些复合体被观察到与膜相关联,而非被抗体聚集。在反复传代后的细胞培养液中也观察到了这些“病毒包”,其包含多达100个病毒粒子。在其他粪便标本中也观察到含有类似细小病毒样颗粒的包。在受粪便污染的水中必然会出现此类复合体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cc/238652/c600cc236ed5/aem00143-0349-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cc/238652/0c0b22efca21/aem00143-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cc/238652/c600cc236ed5/aem00143-0349-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cc/238652/0c0b22efca21/aem00143-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cc/238652/c600cc236ed5/aem00143-0349-a.jpg

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本文引用的文献

1
Further observations on the morphogenesis of human rotavirus in MA 104 cells.关于人轮状病毒在MA 104细胞中形态发生的进一步观察
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A community waterborne gastroenteritis outbreak: evidence for rotavirus as the agent.一起社区水源性肠胃炎暴发:轮状病毒作为病原体的证据。
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Ultrastructural study of rotavirus replication in cultured cells.轮状病毒在培养细胞中复制的超微结构研究。
J Gen Virol. 1980 Jan;46(1):75-85. doi: 10.1099/0022-1317-46-1-75.
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Rapid and reliable method for diagnostic electron microscopy of faeces.粪便诊断电子显微镜检查的快速可靠方法。
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Inactivation of simian rotavirus SA11 by chlorine, chlorine dioxide, and monochloramine.氯、二氧化氯和一氯胺对猴轮状病毒SA11的灭活作用。
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10
Inactivation of human rotavirus, SA11 and other enteric viruses in effluent by disinfectants.消毒剂对污水中人类轮状病毒、SA11及其他肠道病毒的灭活作用。
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