• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在 >100 kHz MAS 下通过质子检测固态 NMR 快速访问 RNA 共振。

Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS.

机构信息

Centre for Biomolecular Drug Research (BMWZ) and Institute of Organic Chemistry, Leibniz University Hannover, Schneiderberg 38, 30167 Hannover, Germany.

Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100 Villeurbanne, France.

出版信息

Chem Commun (Camb). 2018 Aug 21;54(65):8972-8975. doi: 10.1039/c8cc04437f. Epub 2018 Jul 5.

DOI:10.1039/c8cc04437f
PMID:29974085
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6088370/
Abstract

Fast (>100 kHz) magic angle spinning solid-state NMR allows combining high-sensitive proton detection with the absence of an intrinsic molecular weight limit. Using this technique we observe for the first time narrow H RNA resonances and assign nucleotide spin systems with only 200 μg of uniformly C,N-labelled RNA.

摘要

快速 (>100 kHz) 魔角旋转固态 NMR 允许结合高灵敏度的质子检测和不存在固有分子量限制。使用这种技术，我们首次观察到窄 H RNA 共振，并仅使用 200μg 均匀 C,N 标记的 RNA 分配核苷酸自旋系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d393/6088370/b8db2be1e390/c8cc04437f-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d393/6088370/f06067457cc8/c8cc04437f-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d393/6088370/643ed3265812/c8cc04437f-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d393/6088370/b8db2be1e390/c8cc04437f-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d393/6088370/f06067457cc8/c8cc04437f-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d393/6088370/643ed3265812/c8cc04437f-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d393/6088370/b8db2be1e390/c8cc04437f-f3.jpg

相似文献

1
Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS.在 >100 kHz MAS 下通过质子检测固态 NMR 快速访问 RNA 共振。
Chem Commun (Camb). 2018 Aug 21;54(65):8972-8975. doi: 10.1039/c8cc04437f. Epub 2018 Jul 5.
2
Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR.通过魔角旋转固态核磁共振检测纳升体积中完全质子化生物固体的侧链质子共振。
J Biomol NMR. 2018 Mar;70(3):177-185. doi: 10.1007/s10858-018-0168-3. Epub 2018 Mar 3.
3
Proton-detected solid-state NMR spectroscopy of fully protonated proteins at 40 kHz magic-angle spinning.在40千赫兹魔角旋转条件下对完全质子化蛋白质进行质子检测的固态核磁共振光谱学。
J Am Chem Soc. 2007 Sep 26;129(38):11791-801. doi: 10.1021/ja073462m. Epub 2007 Aug 29.
4
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR.超快魔角旋转固态核磁共振中质子总体磁化强度的恢复及灵敏度增强
J Phys Chem B. 2015 Feb 19;119(7):2908-20. doi: 10.1021/jp511987y. Epub 2015 Feb 3.
5
Rapid proton-detected NMR assignment for proteins with fast magic angle spinning.用于快速魔角旋转蛋白质的快速质子检测核磁共振归属
J Am Chem Soc. 2014 Sep 3;136(35):12489-97. doi: 10.1021/ja507382j. Epub 2014 Aug 18.
6
Intermolecular protein-RNA interactions revealed by 2D 31P-15N magic angle spinning solid-state NMR spectroscopy.二维 31P-15N 魔角旋转固态 NMR 光谱揭示的分子间蛋白质-RNA 相互作用。
J Am Chem Soc. 2010 Mar 24;132(11):3842-6. doi: 10.1021/ja909723f.
7
1H, 13C and 15N resonance assignments of the 2'-5' RNA ligase-like protein from Pyrococcus furiosus.嗜热栖热菌2'-5' RNA连接酶样蛋白的1H、13C和15N共振归属
J Biomol NMR. 2006;36 Suppl 1:16. doi: 10.1007/s10858-005-5581-8.
8
Sensitivity and resolution of proton detected spectra of a deuterated protein at 40 and 60 kHz magic-angle-spinning.在40千赫兹和60千赫兹魔角旋转条件下氘代蛋白质的质子检测光谱的灵敏度和分辨率。
J Biomol NMR. 2015 Feb;61(2):161-71. doi: 10.1007/s10858-015-9904-0. Epub 2015 Feb 8.
9
Is protein deuteration beneficial for proton detected solid-state NMR at and above 100 kHz magic-angle spinning?氘代蛋白质是否有利于质子检测的固态 NMR 在 100 kHz 以上魔角旋转？
Solid State Nucl Magn Reson. 2017 Oct;87:126-136. doi: 10.1016/j.ssnmr.2017.07.004. Epub 2017 Jul 25.
10
NMR assignment of the chitin-binding domain of a hyperthermophilic chitinase from Pyrococcus furiosus.嗜热栖热菌嗜热几丁质酶几丁质结合结构域的核磁共振归属
J Biomol NMR. 2006;36 Suppl 1:70. doi: 10.1007/s10858-006-9058-1. Epub 2006 Oct 3.

引用本文的文献

1
Advances in solid-state NMR methods for studying RNA structures and dynamics.用于研究RNA结构与动力学的固态核磁共振方法进展
Magn Reson Lett. 2024 Apr 26;5(1):200133. doi: 10.1016/j.mrl.2024.200133. eCollection 2025 Feb.
2
Proton-Detected Solid-State NMR for Deciphering Structural Polymorphism and Dynamic Heterogeneity of Cellular Carbohydrates in Pathogenic Fungi.用于解析致病真菌中细胞碳水化合物结构多态性和动态异质性的质子检测固态核磁共振技术
J Am Chem Soc. 2025 May 21;147(20):17416-17432. doi: 10.1021/jacs.5c04054. Epub 2025 May 6.
3
Proton-Detected Solid-State NMR for Deciphering Structural Polymorphism and Dynamic Heterogeneity of Cellular Carbohydrates in Pathogenic Fungi.

本文引用的文献

1
H magic-angle spinning NMR evolves as a powerful new tool for membrane proteins.魔角旋转核磁共振成为研究膜蛋白的一种强大的新工具。
J Magn Reson. 2018 Feb;287:140-152. doi: 10.1016/j.jmr.2017.11.014.
2
Efficient low-power TOBSY sequences for fast MAS.用于快速 MAS 的高效低功耗 TOBSY 序列。
Solid State Nucl Magn Reson. 2018 Feb;89:27-34. doi: 10.1016/j.ssnmr.2017.11.003. Epub 2017 Dec 5.
3
Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals.质子检测的固态核磁共振技术可检测微晶中二聚体RNA的核苷酸间相关性和结构。
用于解析致病真菌中细胞碳水化合物结构多态性和动态异质性的质子检测固态核磁共振技术
bioRxiv. 2025 Mar 13:2025.03.09.642223. doi: 10.1101/2025.03.09.642223.
4
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG.用于固态核磁共振灵敏度增强的TOCSY混合评估以及4D实验在全长30 kDa膜蛋白GlpG侧链归属中的应用。
J Biomol NMR. 2025 Mar;79(1):25-34. doi: 10.1007/s10858-024-00454-7. Epub 2025 Jan 22.
5
Dynamics of base pairs with low stability in RNA by solid-state nuclear magnetic resonance exchange spectroscopy.通过固态核磁共振交换光谱法研究RNA中低稳定性碱基对的动力学
iScience. 2022 Oct 10;25(11):105322. doi: 10.1016/j.isci.2022.105322. eCollection 2022 Nov 18.
6
Nucleic acid-protein interfaces studied by MAS solid-state NMR spectroscopy.通过魔角旋转固体核磁共振光谱研究的核酸-蛋白质界面
J Struct Biol X. 2022 Aug 18;6:100072. doi: 10.1016/j.yjsbx.2022.100072. eCollection 2022.
7
H-Detected Biomolecular NMR under Fast Magic-Angle Spinning.在快速魔角旋转下的 H 检测生物分子 NMR。
Chem Rev. 2022 May 25;122(10):9943-10018. doi: 10.1021/acs.chemrev.1c00918. Epub 2022 May 10.
8
NMR Assignment of Methyl Groups in Immobilized Proteins Using Multiple-Bond C Homonuclear Transfers, Proton Detection, and Very Fast MAS.使用多键碳同核转移、质子检测和极快速魔角旋转对固定化蛋白质中的甲基进行核磁共振归属
Front Mol Biosci. 2022 Mar 29;9:828785. doi: 10.3389/fmolb.2022.828785. eCollection 2022.
9
Strategies for RNA Resonance Assignment by C/N- and H-Detected Solid-State NMR Spectroscopy.通过碳/氮和氢检测的固态核磁共振光谱进行RNA共振归属的策略
Front Mol Biosci. 2021 Oct 20;8:743181. doi: 10.3389/fmolb.2021.743181. eCollection 2021.
10
Virus Structures and Dynamics by Magic-Angle Spinning NMR.魔角旋转 NMR 研究病毒结构与动力学
Annu Rev Virol. 2021 Sep 29;8(1):219-237. doi: 10.1146/annurev-virology-011921-064653.
Chem Commun (Camb). 2017 Nov 30;53(96):12886-12889. doi: 10.1039/c7cc07483b.
4
Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization.利用序列侧链相关性和动态核极化（DNP）敏化对完整病毒进行高效分配和核磁共振（NMR）分析。
Proc Natl Acad Sci U S A. 2017 May 16;114(20):5171-5176. doi: 10.1073/pnas.1701484114. Epub 2017 May 1.
5
NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils.NMR 波谱全质子化蛋白质骨架和侧链质子的分配：微晶、沉降组装体和淀粉样纤维。
Angew Chem Int Ed Engl. 2016 Dec 12;55(50):15504-15509. doi: 10.1002/anie.201607084. Epub 2016 Nov 16.
6
Structure of fully protonated proteins by proton-detected magic-angle spinning NMR.通过质子检测魔角旋转核磁共振确定完全质子化蛋白质的结构
Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):9187-92. doi: 10.1073/pnas.1602248113. Epub 2016 Aug 3.
7
Weak and Transient Protein Interactions Determined by Solid-State NMR.通过固态 NMR 确定弱而短暂的蛋白质相互作用。
Angew Chem Int Ed Engl. 2016 Jun 1;55(23):6638-41. doi: 10.1002/anie.201511609. Epub 2016 Apr 21.
8
RNA structure determination by solid-state NMR spectroscopy.通过固态核磁共振光谱法测定RNA结构
Nat Commun. 2015 May 11;6:7024. doi: 10.1038/ncomms8024.
9
Magic angle spinning NMR of proteins: high-frequency dynamic nuclear polarization and (1)H detection.蛋白质的魔角旋转 NMR：高频动态核极化和 (1)H 检测。
Annu Rev Biochem. 2015;84:465-97. doi: 10.1146/annurev-biochem-060614-034206. Epub 2015 Mar 30.
10
Spinning proteins, the faster, the better?旋转蛋白质，转得越快越好？
J Magn Reson. 2015 Apr;253:71-9. doi: 10.1016/j.jmr.2015.01.012.