College of Life Science, Northeast Agricultural University, Harbin 150030, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Molecules. 2018 Jul 6;23(7):1652. doi: 10.3390/molecules23071652.
is a valuable medicinal plant resource with extensive biological activities including anti-cancer, anti-oxidation, and anti-inflammation activities. This work aims to study further the cytotoxic constituents from . In this work, two new phenolic derivatives known as dryofragone () and dryofracoumarin B () with six known compounds (⁻) were isolated from the petroleum ether fraction of the methanol extract of the aerial parts of (L.) Schott by two round cytotoxicity-guided tracking with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2--tetrazolium bromide (MTT) assay and cell counting kit-8 (CCK-8) assay. Their structures were elucidated by the extensive spectroscopic analysis (¹H-NMR, C-NMR, and two dimensions NMR), chemical derivatization, and comparison with data reported in the literature. All the isolates were evaluated for their cytotoxicity against nine cancer cell lines as well as their in vitro immunomodulatory activity. The results showed that compounds have a modest cytotoxicity toward human HeLa cell line with IC value below 30 μM and compounds and may modulate immunity to affect the growth of tumor cells.
是一种具有广泛生物活性的有价值的药用植物资源，包括抗癌、抗氧化和抗炎活性。本工作旨在进一步研究 的细胞毒性成分。在这项工作中，通过 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四唑溴盐（MTT）测定法和细胞计数试剂盒-8（CCK-8）测定法，从 的甲醇提取物的石油醚部分中分离出两种新的酚类衍生物，分别为干呋喃酮（）和干呋喃香豆素 B（），以及六种已知化合物（⁻）。通过两轮细胞毒性导向追踪，它们的结构通过广泛的光谱分析（¹H-NMR、C-NMR 和二维 NMR）、化学衍生化和与文献报道的数据进行比较来阐明。所有分离物均针对其对 9 种癌细胞系的细胞毒性以及体外免疫调节活性进行了评估。结果表明，化合物对人宫颈癌细胞系 HeLa 具有适度的细胞毒性，IC 值低于 30 μM，化合物 和 可能调节免疫以影响肿瘤细胞的生长。
