大肠杆菌HU-2基因的克隆与测序
Cloning and sequencing of the HU-2 gene of Escherichia coli.
作者信息
Kano Y, Osato K, Wada M, Imamoto F
机构信息
Laboratory of Molecular Genetics, (Riken) Tsukuba Life Science Center, Ibaraki, Japan.
出版信息
Mol Gen Genet. 1987 Sep;209(2):408-10. doi: 10.1007/BF00329674.
The Escherichia coli HU-2 gene was cloned using a DNA fragment from the HU-1 gene as a probe. The amino acid sequence of the HU-2 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream of the translation initiation codon (AUG) and a possible rho-independent terminater site downstream of the termination codon (UAA) of the gene.
利用来自HU-1基因的DNA片段作为探针克隆了大肠杆菌HU-2基因。从核苷酸序列推导的HU-2蛋白的氨基酸序列与已发表的序列高度一致。该核苷酸序列在翻译起始密码子（AUG）上游有一个可能的启动子和一个典型的核糖体结合位点，在该基因的终止密码子（UAA）下游有一个可能的不依赖于ρ因子的终止位点。
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