• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

大肠杆菌HU-2基因的克隆与测序

Cloning and sequencing of the HU-2 gene of Escherichia coli.

作者信息

Kano Y, Osato K, Wada M, Imamoto F

机构信息

Laboratory of Molecular Genetics, (Riken) Tsukuba Life Science Center, Ibaraki, Japan.

出版信息

Mol Gen Genet. 1987 Sep;209(2):408-10. doi: 10.1007/BF00329674.

DOI:10.1007/BF00329674
PMID:3312963
Abstract

The Escherichia coli HU-2 gene was cloned using a DNA fragment from the HU-1 gene as a probe. The amino acid sequence of the HU-2 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream of the translation initiation codon (AUG) and a possible rho-independent terminater site downstream of the termination codon (UAA) of the gene.

摘要

利用来自HU-1基因的DNA片段作为探针克隆了大肠杆菌HU-2基因。从核苷酸序列推导的HU-2蛋白的氨基酸序列与已发表的序列高度一致。该核苷酸序列在翻译起始密码子(AUG)上游有一个可能的启动子和一个典型的核糖体结合位点,在该基因的终止密码子(UAA)下游有一个可能的不依赖于ρ因子的终止位点。

相似文献

1
Cloning and sequencing of the HU-2 gene of Escherichia coli.大肠杆菌HU-2基因的克隆与测序
Mol Gen Genet. 1987 Sep;209(2):408-10. doi: 10.1007/BF00329674.
2
Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli.大肠杆菌HU-1基因的分子克隆及核苷酸序列
Mol Gen Genet. 1985;201(2):360-2. doi: 10.1007/BF00425687.
3
Cloning and sequencing of the gene for the DNA-binding 17K protein of Escherichia coli.
Gene. 1988 Jul 15;67(1):117-24. doi: 10.1016/0378-1119(88)90014-5.
4
Alcohol dehydrogenase gene from Alcaligenes eutrophus: subcloning, heterologous expression in Escherichia coli, sequencing, and location of Tn5 insertions.嗜中温产碱杆菌的乙醇脱氢酶基因:亚克隆、在大肠杆菌中的异源表达、测序及Tn5插入位点定位
J Bacteriol. 1988 Nov;170(11):5248-56. doi: 10.1128/jb.170.11.5248-5256.1988.
5
Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli.大肠杆菌定居因子抗原I基因的分子克隆及核苷酸序列
Infect Immun. 1989 Apr;57(4):1126-30. doi: 10.1128/iai.57.4.1126-1130.1989.
6
Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12.大肠杆菌K-12无机焦磷酸酶编码基因的克隆与特性分析
J Bacteriol. 1988 Dec;170(12):5901-7. doi: 10.1128/jb.170.12.5901-5907.1988.
7
Nucleotide sequence of the gene for Escherichia coli ribosomal protein S15 (rpsO).大肠杆菌核糖体蛋白S15(rpsO)基因的核苷酸序列。
Mol Gen Genet. 1984;197(2):225-9. doi: 10.1007/BF00330967.
8
Cloning and nucleotide sequencing of the genes for ribosomal proteins S9 (rpsI) and L13 (rplM) of Escherichia coli.大肠杆菌核糖体蛋白S9(rpsI)和L13(rplM)基因的克隆与核苷酸测序
Mol Gen Genet. 1985;198(2):279-82. doi: 10.1007/BF00383007.
9
Escherichia coli RNase D: sequencing of the rnd structural gene and purification of the overexpressed protein.大肠杆菌核糖核酸酶D:rnd结构基因的测序及过表达蛋白的纯化
Nucleic Acids Res. 1988 Jul 25;16(14A):6265-78. doi: 10.1093/nar/16.14.6265.
10
Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene.大肠杆菌K-12 ackA基因的克隆、表达及核苷酸序列
J Bacteriol. 1989 Jan;171(1):577-80. doi: 10.1128/jb.171.1.577-580.1989.

引用本文的文献

1
The Role of the Environment in Horizontal Gene Transfer.环境在水平基因转移中的作用。
Mol Biol Evol. 2022 Nov 3;39(11). doi: 10.1093/molbev/msac220.
2
HU content and dynamics in Escherichia coli during the cell cycle and at different growth rates.大肠杆菌在细胞周期及不同生长速率下的HU含量与动态变化
FEMS Microbiol Lett. 2017 Oct 16;364(19). doi: 10.1093/femsle/fnx195.
3
Molecular Mechanisms of Transcription Initiation at gal Promoters and their Multi-Level Regulation by GalR, CRP and DNA Loop.半乳糖启动子转录起始的分子机制及其受GalR、CRP和DNA环的多级调控

本文引用的文献

1
Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli.来自大肠杆菌的DNA结合蛋白HU-1和HU-2的完整氨基酸序列。
Eur J Biochem. 1980 Feb;103(3):447-61. doi: 10.1111/j.1432-1033.1980.tb05968.x.
2
Isolation and characterization of nucleoid proteins from Escherichia coli.大肠杆菌类核蛋白的分离与特性分析
Mol Gen Genet. 1984;196(2):217-24. doi: 10.1007/BF00328053.
3
Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli.蛋白质HU在大肠杆菌染色体复制起点的酶促复制过程中的作用
Biomolecules. 2015 Oct 16;5(4):2782-807. doi: 10.3390/biom5042782.
4
The SOS Regulatory Network.SOS调控网络。
EcoSal Plus. 2008 Jul 25;2008. doi: 10.1128/ecosalplus.5.4.3.
5
The regulation of HanA during heterocyst development in cyanobacterium Anabaena sp. PCC 7120.蓝藻鱼腥藻PCC 7120异形胞发育过程中HanA的调控。
World J Microbiol Biotechnol. 2014 Oct;30(10):2673-80. doi: 10.1007/s11274-014-1691-3. Epub 2014 Jul 1.
6
The histone-like protein HU does not obstruct movement of T7 RNA polymerase in Escherichia coli cells but stimulates its activity.类组蛋白HU并不阻碍T7 RNA聚合酶在大肠杆菌细胞中的移动,反而会刺激其活性。
J Bacteriol. 2002 Mar;184(6):1565-70. doi: 10.1128/JB.184.6.1565-1570.2002.
7
Involvement of H-NS in transpositional recombination mediated by IS1.H-NS参与由IS1介导的转座重组。
J Bacteriol. 2001 Apr;183(8):2476-84. doi: 10.1128/JB.183.8.2476-2484.2001.
8
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱,第10版:传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
9
Alterations of the outer membrane composition in Escherichia coli lacking the histone-like protein HU.缺乏类组蛋白HU的大肠杆菌外膜成分的改变
Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6712-7. doi: 10.1073/pnas.94.13.6712.
10
The Escherichia coli histone-like protein HU affects DNA initiation, chromosome partitioning via MukB, and cell division via MinCDE.大肠杆菌类组蛋白HU影响DNA起始、通过MukB进行的染色体分配以及通过MinCDE进行的细胞分裂。
J Bacteriol. 1997 Jun;179(11):3494-9. doi: 10.1128/jb.179.11.3494-3499.1997.
Proc Natl Acad Sci U S A. 1984 Jan;81(2):424-8. doi: 10.1073/pnas.81.2.424.
4
Codon usage and transfer RNA contents: organism-specific codon-choice patterns in reference to the isoacceptor contents.密码子使用与转运RNA含量:参照同功受体含量的物种特异性密码子选择模式。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1087-97. doi: 10.1101/sqb.1983.047.01.123.
5
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒,一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
6
The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex.在蛋白质 - DNA - RNA 复合物中分离出的大肠杆菌折叠基因组。
Proc Natl Acad Sci U S A. 1971 Jan;68(1):6-9. doi: 10.1073/pnas.68.1.6.
7
Replication of the Escherichia coli chromosome with a soluble enzyme system.用可溶性酶系统进行大肠杆菌染色体的复制。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3189-93. doi: 10.1073/pnas.71.8.3189.
8
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.大肠杆菌16S核糖体RNA的3'末端序列:与无义三联体及核糖体结合位点的互补性
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6. doi: 10.1073/pnas.71.4.1342.
9
On the structure of the folded chromosome of Escherichia coli.关于大肠杆菌折叠染色体的结构
J Mol Biol. 1972 Nov 14;71(2):127-47. doi: 10.1016/0022-2836(72)90342-7.
10
Genetic characterization of the gene hupB encoding the HU-1 protein of Escherichia coli.编码大肠杆菌HU-1蛋白的hupB基因的遗传特征分析
Gene. 1986;45(1):37-44. doi: 10.1016/0378-1119(86)90129-0.