Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands.
Department of Nutrition and Movement Sciences, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands.
Scand J Med Sci Sports. 2019 Feb;29(2):213-222. doi: 10.1111/sms.13324. Epub 2018 Nov 12.
This study aimed to analyze the intra-individual variation in VO of human subjects using total-capture and free-flow indirect calorimetry. Twenty-seven men (27 ± 5 year; VO 49-79 mL•kg •min ) performed two maximal exertion tests (CPETs) on a cycle ergometer, separated by a 7 ± 2 day interval. VO and VCO were assessed using an indirect calorimeter (Omnical) with total capture of exhalation in a free-flow airstream. Thirteen subjects performed a third maximal exertion test using a breath-by-breath calorimeter (Oxycon Pro). On-site validation was deemed a requirement. For the Omnical, the mean within-subject CV for VO was 1.2 ± 0.9% (0.0%-4.4%) and for ergometer workload P 1.3 ± 1.3% (0%-4.6%). VO values with the Oxycon Pro were significantly lower in comparison with Omnical (P < 0.001; t test) with mean 3570 vs 4061 and difference SD 361 mL•min . Validation results for the Omnical with methanol combustion were -0.05 ± 0.70% (mean ± SD; n = 31) at the 225 mL•min VO level and -0.23 ± 0.80% (n = 31) at the 150 mL•min VCO level. Results using gas infusion were 0.04 ± 0.75% (n = 34) and -0.99 ± 1.05% (n = 24) over the respective 500-6000 mL•min VO and VCO ranges. Validation results for the Oxycon Pro in breath-by-breath mode were - 2.2 ± 1.6% (n = 12) for VO and 5.7 ± 3.3% (n = 12) for VCO over the 1000-4000 mL•min range. On a Visual analog scale, participants reported improved breathing using the free-flow indirect calorimetry (score 7.6 ± 1.2 vs 5.1 ± 2.7, P = 0.008). We conclude that total capturing free-flow indirect calorimetry is suitable for measuring VO even with the highest range. VO was linear with the incline in P over the full range.
本研究旨在使用全捕获和自由流动间接测热法分析人体受试者的个体内变异。27 名男性（27±5 岁；VO 49-79mL•kg •min ）在自行车测力计上进行了两次最大用力测试（CPET），间隔 7±2 天。使用间接测热仪（Omnical）以自由流动气流中的呼气全捕获来评估 VO 和 VCO。13 名受试者使用逐口气流热量计（Oxycon Pro）进行了第三次最大用力测试。现场验证被认为是必需的。对于 Omnical，VO 的个体内 CV 平均值为 1.2±0.9%（0.0%-4.4%），对于测力计工作负载 P 为 1.3±1.3%（0%-4.6%）。与 Omnical 相比，Oxycon Pro 的 VO 值明显较低（P<0.001；t 检验），平均值分别为 3570 与 4061，差异标准差为 361mL•min 。用甲醇燃烧对 Omnical 的验证结果为在 225mL•min VO 水平为-0.05±0.70%（平均值±标准差；n=31），在 150mL•min VCO 水平为-0.23±0.80%（n=31）。气体输注的结果分别为 0.04±0.75%（n=34）和-0.99±1.05%（n=24），在各自的 500-6000mL•min VO 和 VCO 范围内。在逐口气流模式下，Oxycon Pro 的验证结果为 VO 为-2.2±1.6%（n=12），VCO 为 5.7±3.3%（n=12），在 1000-4000mL•min 范围内。在视觉模拟量表上，参与者报告使用自由流动间接测热法改善了呼吸（评分 7.6±1.2 与 5.1±2.7，P=0.008）。我们得出结论，即使在最高范围内，全捕获自由流动间接测热法也适合测量 VO。VO 与 P 的斜率呈线性关系，在整个范围内。
