Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Mechanical and Aerospace Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
J Mol Neurosci. 2019 Jan;67(1):48-61. doi: 10.1007/s12031-018-1209-9. Epub 2018 Nov 27.
Motor and sensory recovery following critical size peripheral nerve defects is often incomplete. Although nerve grafting has been proposed as the gold standard, it is associated with several disadvantages. Here we report a novel approach to peripheral nerve repair using Human Unrestricted Somatic Stem Cells (USSC) delivered through an electrospun neural guidance conduit. Conduits were produced from PCL and gelatin blend. Several in vitro methods were utilized to investigate the conduit's physicochemical and biological characteristics. Nerve regeneration was studied across a 10-mm sciatic nerve gap in Wistar rats. For functional analysis, the conduits were seeded with 3 × 10 USSCs and implanted into a 10-mm sciatic nerve defect. After 14 weeks, the results of functional recovery analysis and histopathological examinations showed that animals implanted with USSC containing conduits exhibited improved functional and histopathological recovery which was more close to the autograft group compared to other groups. Our results support the potential applicability of USSCs to treat peripheral nerve injury in the clinic.
在临界尺寸周围神经缺损后，运动和感觉功能的恢复往往是不完全的。虽然神经移植被认为是金标准，但它存在一些缺点。在这里，我们报告了一种使用人无限制体干细胞（USSC）通过电纺神经引导导管修复周围神经的新方法。导管由 PCL 和明胶混合物制成。利用多种体外方法研究了导管的理化和生物学特性。在 Wistar 大鼠的 10mm 坐骨神经间隙中研究了神经再生。为了进行功能分析，将 3×10 个 USSC 接种到神经导管中，并植入 10mm 坐骨神经缺损处。14 周后，功能恢复分析和组织病理学检查的结果表明，与其他组相比，植入含 USSC 的导管的动物表现出更好的功能和组织病理学恢复，更接近自体移植物组。我们的结果支持 USSC 在临床上治疗周围神经损伤的应用潜力。
