Kruppke Benjamin, Heinemann Christiane, Wagner Alena-Svenja, Farack Jana, Wenisch Sabine, Wiesmann Hans-Peter, Hanke Thomas
Max Bergmann Center of Biomaterials and Institute of Materials Science, Technische Universität Dresden, Dresden, Germany.
Clinic of Small Animals, c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Justus-Liebig-University Gießen, Gießen, Germany.
Dev Growth Differ. 2019 Feb;61(2):166-175. doi: 10.1111/dgd.12588. Epub 2018 Dec 25.
In order to investigate the influence of calcium and strontium ion concentration on human bone marrow stromal cells and their differentiation to osteoblasts, different cell culture media have been used. Even though this study does not contain a bone substitute material, the reason for this study was the decrease of cation concentration by many biomaterials, due to induced apatite precipitation. As a consequence, the reduced calcium ion concentration is known to affect osteoblastic development. Therefore, the main focus was put on the question, whether an increased strontium concentration (in the range of mM) might be suitable to compensate the lack of calcium ions. The effect of solely strontium ions-with only calcium in the media resulting from fetal calf serum-was investigated. Commercially available calcium-free medium (modified α-MEM) was tested in comparison with media with varied calcium ion concentrations (0.9, 1.8, and 3.6 mM), or strontium ion concentration (0.4, 0.9, 1.8, and 3.6 mM). In case of calcium, higher concentrations cause increased proliferation, while differentiation was shifted to earlier points of time. Differentiation was increased by solely strontium ions only at 0.4-0.9 mM, while proliferation was highest for 0.9-1.8 mM. From these results, it can be concluded that strontium is able to compensate a lack of calcium to a certain degree. Thus, in contrast to calcium ion release, a strontium ion release from bone substitute materials might be applicable for stimulation of bone regeneration without influencing the media saturation.
为了研究钙和锶离子浓度对人骨髓基质细胞及其向成骨细胞分化的影响，使用了不同的细胞培养基。尽管本研究未包含骨替代材料，但进行该研究的原因是许多生物材料会因诱导磷灰石沉淀而导致阳离子浓度降低。因此，已知钙离子浓度降低会影响成骨细胞的发育。所以，主要关注的问题是，增加的锶浓度（在毫摩尔范围内）是否可能适合补偿钙离子的缺乏。研究了仅含锶离子（培养基中仅来自胎牛血清的钙）的效果。将市售的无钙培养基（改良α-MEM）与钙离子浓度不同（0.9、1.8和3.6毫摩尔）或锶离子浓度不同（0.4、0.9、1.8和3.6毫摩尔）的培养基进行了比较。就钙而言，较高浓度会导致增殖增加，而成骨分化则提前。仅在0.4 - 0.9毫摩尔时，仅锶离子会增加成骨分化，而在0.9 - 1.8毫摩尔时增殖最高。从这些结果可以得出结论，锶能够在一定程度上补偿钙的缺乏。因此，与钙离子释放不同，骨替代材料释放锶离子可能适用于刺激骨再生而不影响培养基饱和度。
