一种实验性硅酸钙基根管封闭剂的细胞相容性、生物活性潜力和抗菌活性。

Cytocompatibility, bioactive potential and antimicrobial activity of an experimental calcium silicate-based endodontic sealer.

机构信息

Department of Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil.

出版信息

Int Endod J. 2019 Jul;52(7):979-986. doi: 10.1111/iej.13086. Epub 2019 Feb 18.

DOI:10.1111/iej.13086

PMID:30702145

Abstract

AIM

To evaluate the cytocompatibility, bioactive potential, antimicrobial and antibiofilm activities of an experimental calcium silicate-based endodontic sealer, in comparison with TotalFill BC Sealer and AH Plus.

METHODOLOGY

Cytocompatibility was assessed by methyltetrazolium (MTT) and neutral red (NR) assays, after exposure of the Saos-2 cells to the sealer extracts (1 : 2, 1 : 4, 1 : 8, 1 : 16 and 1 : 32 dilutions) for 24 h. The sealers were manipulated and placed in 12-well culture plates and exposed to ultraviolet light; then, 5 mL of DMEM without serum was added. Cell bioactivity was evaluated by alkaline phosphatase activity (ALP) and Alizarin red staining (ARS). Antimicrobial and antibiofilm activities were evaluated by direct contact test (DCT) on planktonic cells (DCTPC) and modified DCT on biofilm formed in bovine dentine blocks (MDCT). MTT, NR and ALP data were analysed by two-way anova and Bonferroni tests; ARS data by anova and Tukey's tests; and the microbiological data by Kruskal-Wallis and Dunn tests (α = 0.05).

RESULTS

The experimental sealer, TotalFill BC and AH Plus were not cytotoxic to Saos-2, in comparison with the negative control (P > 0.05). Greater ALP was observed after 7 days of exposure of Saos-2 to AH Plus and the experimental sealer (P < 0.05) when compared to the control. Significantly greater mineralized nodule production was observed for TotalFill BC and the experimental sealer (P < 0.05). In DCTPC, the experimental sealer and TotalFill BC were associated with a significantly greater reduction of E. faecalis (P < 0.05) and eliminated C. albicans. In MDCT, the experimental sealer and TotalFill BC had significantly greater antibiofilm efficacy (P < 0.05).

CONCLUSIONS

The experimental calcium silicate-based sealer was cytocompatible, bioactive, antimicrobial against E. faecalis and C. albicans and effective against E. faecalis biofilms, with potential for use in root canal treatment.

摘要

目的

评估一种实验性钙硅酸钠基根管封闭剂的细胞相容性、生物活性、抗菌和抗生物膜活性，并与 TotalFill BC 封闭剂和 AH Plus 进行比较。

方法

将 Saos-2 细胞暴露于封闭剂提取物（1：2、1：4、1：8、1：16 和 1：32 稀释液）24 小时后，通过甲基噻唑基四唑（MTT）和中性红（NR）测定评估细胞相容性。将封闭剂进行操作并放置在 12 孔培养板中，然后暴露于紫外线下；然后，加入 5 mL 无血清 DMEM。通过碱性磷酸酶活性（ALP）和茜素红染色（ARS）评估细胞的生物活性。通过浮游细胞直接接触试验（DCTPC）和改良的牛牙本质块生物膜 DCT（MDCT）评估抗菌和抗生物膜活性。通过双向方差分析和 Bonferroni 检验分析 MTT、NR 和 ALP 数据；通过方差分析和 Tukey 检验分析 ARS 数据；通过 Kruskal-Wallis 和 Dunn 检验分析微生物数据（α=0.05）。

结果

与阴性对照相比（P>0.05），实验性封闭剂、TotalFill BC 和 AH Plus 对 Saos-2 无细胞毒性。与对照组相比，Saos-2 暴露于 AH Plus 和实验性封闭剂 7 天后，ALP 显著升高（P<0.05）。与 TotalFill BC 和实验性封闭剂相比，观察到显著更多的矿化结节生成（P<0.05）。在 DCTPC 中，实验性封闭剂和 TotalFill BC 与显著降低粪肠球菌（P<0.05）和消除白色念珠菌相关。在 MDCT 中，实验性封闭剂和 TotalFill BC 具有显著更高的抗生物膜效果（P<0.05）。