Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.
Faculty of Fisheries, İzmir Katip Çelebi University, Çiğli, 35620 İzmir, Turkey.
Mar Drugs. 2019 Feb 3;17(2):99. doi: 10.3390/md17020099.
The fungus was isolated from the Mediterranean sponge . The initial fermentation of the fungus on solid rice medium yielded 16 known compounds (⁻). The addition of several inorganic salts to the rice medium mainly influenced the accumulation of these secondary metabolites. Fermentation of the fungus on white bean medium yielded the new waspergillamide B () featuring an unusual -nitrobenzoic acid as partial structure. Moreover, two new compounds, ochraspergillic acids A and B ( and ), which are both adducts of dihydropenicillic acid and - or -aminobenzoic acid, were isolated from the co-culture of the fungus with . Compound was also detected in axenic fungal cultures following the addition of either anthranilic acid or tryptophan to the rice medium. The structures of the new compounds were established by 1D and 2DNMR experiments as well as from the HRMS data. The absolute configuration of was elucidated following hydrolysis and derivatization of the amino acids using Marfey's reagent. Viomellein () and ochratoxin B () exhibited strong cytotoxicity against the A2780 human ovarian carcinoma cells with IC values of 5.0 and 3.0 M, respectively.
该真菌从地中海海绵中分离得到。真菌在固体大米培养基上的初始发酵得到 16 种已知化合物（⁻）。向大米培养基中添加几种无机盐主要影响这些次生代谢物的积累。真菌在白豆培养基上的发酵得到了新型沃氏青霉素 B（），其具有不寻常的 -硝基苯甲酸作为部分结构。此外，从真菌与的共培养物中分离得到了两种新化合物，ochraspergillic 酸 A 和 B（和），它们都是二氢青霉酸和 -或-氨基苯甲酸的加合物。在向大米培养基中添加邻氨基苯甲酸或色氨酸后，在无菌真菌培养物中也检测到了化合物。新化合物的结构通过 1D 和 2D NMR 实验以及 HRMS 数据确定。通过使用 Marfey 试剂对氨基酸进行水解和衍生化，确定了的绝对构型。viomellein（）和 ochratoxin B（）对 A2780 人卵巢癌细胞表现出强烈的细胞毒性，IC 值分别为 5.0 和 3.0 M。
