Wahl Daniel J, Ng Ringo, Ju Myeong Jin, Jian Yifan, Sarunic Marinko V
Engineering Science, Simon Fraser University, Burnaby, BC, Canada.
Casey Eye Institute, Oregon Health & Science University, Portland, OR, USA.
Biomed Opt Express. 2018 Dec 19;10(1):252-267. doi: 10.1364/BOE.10.000252. eCollection 2019 Jan 1.
Vision researchers often use small animals due to the availability of many transgenic strains that model human diseases or express biomarkers. Adaptive optics (AO) enables non-invasive single-cell imaging in a living animal but often results in high system complexity. Sensorless AO (SAO) can provide depth-resolved aberration correction with low system complexity. We present a multi-modal sensorless AO retina imaging system that includes optical coherence tomography (OCT), OCT-angiography, confocal scanning laser ophthalmoscopy (SLO), and fluorescence detection. We present a compact lens-based imaging system design that allows for a 50-degree maximum field of view (FOV), which can be reduced to the region of interest to perform SAO with the modality of choice. The system performance was demonstrated on wild type mice (C57BL/6J), and transgenic mice with GFP labeled cells. SAO SLO was used for imaging microglia (Cx3cr1-GFP) over ~1 hour, where dynamics of the microglia branches were clearly observed. Our results also include volumetric cellular imaging of microglia throughout the inner retina.
视觉研究人员经常使用小型动物，因为有许多可模拟人类疾病或表达生物标志物的转基因品系。自适应光学（AO）能够在活体动物中进行非侵入性单细胞成像，但往往会导致系统复杂性较高。无传感器AO（SAO）可以在低系统复杂性的情况下提供深度分辨的像差校正。我们展示了一种多模态无传感器AO视网膜成像系统，该系统包括光学相干断层扫描（OCT）、OCT血管造影、共焦扫描激光检眼镜（SLO）和荧光检测。我们展示了一种基于透镜的紧凑型成像系统设计，其最大视野（FOV）为50度，可缩小到感兴趣区域，以便使用所选模态执行SAO。该系统性能在野生型小鼠（C57BL/6J）和带有绿色荧光蛋白标记细胞的转基因小鼠上得到了验证。SAO SLO用于对小胶质细胞（Cx3cr1-GFP）进行约1小时的成像，在此期间清晰观察到了小胶质细胞分支的动态变化。我们的结果还包括对整个内视网膜小胶质细胞的体积细胞成像。
