使用市售干式微量稀释板进行亚胺培南药敏试验。
Imipenem susceptibility testing with a commercially prepared dry-format microdilution tray.
作者信息
Staneck J L
出版信息
J Clin Microbiol. 1986 Jun;23(6):1134-5. doi: 10.1128/jcm.23.6.1134-1135.1986.
Abstract
MICs of imipenem, concurrently generated in commercially prepared microdilution trays containing predried antibiotic dilutions (Sensititre), and in a standard agar dilution assay (as recommended by the National Committee for Clinical Laboratory Standards, Villanova, Pa.), were within +/- 1 twofold dilution for 94% of 226 bacterial isolates. Imipenem biological activity remained stable over 5 months of tray storage at room temperature against Pseudomonas aeruginosa ATCC 27853. Activity of imipenem was shown by microdilution testing with 890 clinical isolates to be high, with only 4% of isolates having MICs of greater than or equal to 16 micrograms/ml (in vitro resistance).
摘要
亚胺培南的最低抑菌浓度（MIC），同时在含有预干燥抗生素稀释液的商业制备微量稀释板（Sensititre）中以及在标准琼脂稀释试验（按照宾夕法尼亚州维拉诺瓦市临床实验室标准委员会的推荐）中测定，对于226株细菌分离株中的94%，二者结果在±1倍稀释范围内。在室温下将微量稀释板保存5个月期间，亚胺培南对铜绿假单胞菌ATCC 27853的生物活性保持稳定。通过对890株临床分离株进行微量稀释试验表明，亚胺培南的活性较高，只有4%的分离株的MIC大于或等于16微克/毫升（体外耐药）。
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