Flegkas Alexandros, Milosević Ifantis Tanja, Barda Christina, Samara Pinelopi, Tsitsilonis Ourania, Skaltsa Helen
Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece.
Department of Biology, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15784 Athens, Greece.
Medicines (Basel). 2019 Mar 12;6(1):37. doi: 10.3390/medicines6010037.
L. (holy basil; Tulsi in Hindi) is an important medicinal plant, traditionally used in India. The phytochemical study of the nonpolar (dichloromethane 100%) and polar (methanol:water; 7:3) extracts yielded fourteen compounds. Compounds , , , , , and , along with the methanol:water extract were evaluated for their cytotoxicity against the human cancer cell lines MCF-7, SKBR3, and HCT-116, and normal peripheral blood mononuclear cells (PBMCs). Five terpenoids, namely, ursolic acid (), oleanolic acid (), betulinic acid (), stigmasterol (), and -caryophyllene oxide (); two lignans, i.e., (-)-rabdosiin () and shimobashiric acid C (); three flavonoids, luteolin (), its 7---D-glucuronide (), apigenin 7---D-glucuronide (); and four phenolics, (-p-coumaroyl 4---D-glucoside (), 3-(3,4-dihydroxyphenyl) lactic acid (), protocatechuic acid (), and vanillic acid () were isolated. Compound was the most cytotoxic against the human cancer lines assessed and showed very low cytotoxicity against PBMCs. Based on these results, the structure of compound shows some promise as a selective anticancer drug scaffold.
罗勒（圣罗勒；印地语称图尔西）是一种重要的药用植物，在印度传统上被使用。对非极性（100%二氯甲烷）和极性（甲醇:水；7:3）提取物的植物化学研究产生了14种化合物。对化合物1、2、3、4、5、6以及甲醇:水提取物针对人癌细胞系MCF - 7、SKBR3和HCT - 116以及正常外周血单个核细胞（PBMCs）的细胞毒性进行了评估。分离出了5种萜类化合物，即熊果酸（1）、齐墩果酸（2）、桦木酸（3）、豆甾醇（4）和氧化石竹烯（5）；2种木脂素，即（-）-香茶菜素（6）和岛叶酸C（7）；3种黄酮类化合物，木犀草素（8）、其7 - O - D -葡萄糖醛酸苷（9）、芹菜素7 - O - D -葡萄糖醛酸苷（10）；以及4种酚类化合物，对香豆酰4 - O - D -葡萄糖苷（11）、3 -（3,4 -二羟基苯基）乳酸（12）、原儿茶酸（13）和香草酸（14）。化合物1对所评估的人癌细胞系细胞毒性最强，对PBMCs显示出非常低的细胞毒性。基于这些结果，化合物1的结构显示出作为一种选择性抗癌药物支架的一些前景。
