Laboratorio de Investigación en Genómica, Genética y Bioinformática. Torre de Hemato-Oncología, 4to Piso, Sección 2. Hospital Infantil de México, Federico Gómez.
Nanomedicine. 2019 Jun;18:428-431. doi: 10.1016/j.nano.2019.03.006. Epub 2019 Mar 30.
Recently Jennifer Doudna's group discovered the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated protein 14 (Cas14), identified almost exclusively in a superphylum of extremophile archaea. The newly discovered Cas14 possesses a single-stranded (ss)DNA targeting activity - despite being two times smaller than Cas9 - a capability that might confer a defense against viruses with ssDNA genomes. Furthermore, by combining the non-specific ssDNase cleavage activity of Cas14 with isothermal amplification method (DETECTR-Cas14), it can also be promisingly exploited for high-fidelity DNA single-nucleotide polymorphism genotyping, and potentially for detecting ssDNA viruses of undeniable clinical, ecological, and economic importance infecting hosts in all three domains of life. Thus, CRISPR-Cas14 might acquire an exponential expansion in the field of CRISPR diagnostic for infectious and noninfectious diseases.
最近，Jennifer Doudna 的团队发现了 CRISPR-CRISPR 相关蛋白 14（Cas14），它几乎只存在于超嗜极古菌的一个超级门中。新发现的 Cas14 具有单链 DNA 靶向活性——尽管它比 Cas9 小两倍——这种能力可能赋予了针对具有 ssDNA 基因组的病毒的防御能力。此外，通过将 Cas14 的非特异性单链 DNA 切割活性与等温扩增方法（DETECTR-Cas14）相结合，它也有望用于高保真度的 DNA 单核苷酸多态性基因分型，并且可能用于检测具有不可否认的临床、生态和经济重要性的 ssDNA 病毒感染所有三个生命领域的宿主。因此，CRISPR-Cas14 在用于传染病和非传染病的 CRISPR 诊断领域可能会呈指数级扩展。
