Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus.
作者信息
Shepherd A J, Swanepoel R, Leman P A, Shepherd S P
出版信息
J Clin Microbiol. 1986 Oct;24(4):654-6. doi: 10.1128/jcm.24.4.654-656.1986.
Abstract
The fluorescence focus assay and the plaque assay in CER cells were compared with mouse inoculation for the isolation and titration of Crimean-Congo hemorrhagic fever virus. The fluorescence focus assay and the plaque assay were of similar sensitivity, but both produced 10- to 100-fold lower titers than did mouse inoculation. For specimens from 26 Crimean-Congo hemorrhagic fever patients in South Africa, virus was isolated from 20 by mouse inoculation and from only 11 by cell culturing. Although cell cultures were less sensitive for the isolation of virus from clinical specimens, they produced diagnostic results much more rapidly.
摘要
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本文引用的文献
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Nosocomial outbreak of viral hemorrhagic fever caused by Crimean Hemorrhagic fever-Congo virus in Pakistan, January 1976.1976年1月，巴基斯坦发生由克里米亚-刚果出血热病毒引起的医院内病毒性出血热暴发。
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