Shepherd A J, Swanepoel R, Leman P A, Shepherd S P
J Clin Microbiol. 1986 Oct;24(4):654-6. doi: 10.1128/jcm.24.4.654-656.1986.
The fluorescence focus assay and the plaque assay in CER cells were compared with mouse inoculation for the isolation and titration of Crimean-Congo hemorrhagic fever virus. The fluorescence focus assay and the plaque assay were of similar sensitivity, but both produced 10- to 100-fold lower titers than did mouse inoculation. For specimens from 26 Crimean-Congo hemorrhagic fever patients in South Africa, virus was isolated from 20 by mouse inoculation and from only 11 by cell culturing. Although cell cultures were less sensitive for the isolation of virus from clinical specimens, they produced diagnostic results much more rapidly.
将克里米亚-刚果出血热病毒分离和滴定的荧光灶试验及空斑试验在CER细胞中进行，并与小鼠接种法作比较。荧光灶试验和空斑试验的灵敏度相似，但二者产生的滴度均比小鼠接种法低10至100倍。对于来自南非26例克里米亚-刚果出血热患者的标本，通过小鼠接种法从20例中分离出病毒，而通过细胞培养仅从11例中分离出病毒。虽然细胞培养对于从临床标本中分离病毒的敏感性较低，但它们能更快地得出诊断结果。
