Saluzzo J F, Le Guenno B
J Clin Microbiol. 1987 May;25(5):922-4. doi: 10.1128/jcm.25.5.922-924.1987.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect Crimean-Congo hemorrhagic fever (CCHF) virus-specific immunoglobulin M (IgM) in human serum samples. For this test, a heat-inactivated antigen was prepared from the brains of suckling mice infected with CCHF virus. The IgM-capture ELISA proved more sensitive than indirect fluorescence tests for IgM to this virus. A human serum containing high-titer IgM to CCHF virus was used for an antigen-capture ELISA to detect this virus in heat-inactivated suspensions of virus-infected ticks. The antigen-capture ELISA appeared to be as sensitive as virus isolation in suckling mice. The studies described suggest that the IgM-capture ELISA and the antigen-detection ELISA should provide a rapid and sensitive diagnosis of human CCHF virus infection and should be useful in ecologic studies of this virus.
开发了一种酶联免疫吸附测定（ELISA）法，用于检测人血清样本中克里米亚-刚果出血热（CCHF）病毒特异性免疫球蛋白M（IgM）。对于该检测，从感染CCHF病毒的乳鼠脑中制备热灭活抗原。IgM捕获ELISA法对该病毒IgM的检测比间接荧光试验更灵敏。使用含有高滴度CCHF病毒IgM的人血清进行抗原捕获ELISA，以检测病毒感染蜱的热灭活悬液中的该病毒。抗原捕获ELISA法似乎与乳鼠病毒分离法一样灵敏。所述研究表明，IgM捕获ELISA法和抗原检测ELISA法应能对人类CCHF病毒感染进行快速灵敏的诊断，且应有助于该病毒的生态学研究。
