School of Pharmaceutical Science and Technology, Health Sciences Platform, Tianjin University,Tianjin 30072, China.
National Herbarium of Georgia, Ilia State University, Tbilisi 100995, Georgia.
Molecules. 2019 Apr 19;24(8):1561. doi: 10.3390/molecules24081561.
A phytochemical investigation of the whole plant of led to the isolationof one previously undescribed labdane diterpenoid, (4,5,9,10)-13-des-ethyl-13-oxolabda-8(17),11-dien-19-oic acid (), together with nine known diterpenoids (-, -), two lignans (, ),and a coumarin (). The structures of all the compounds were elucidated on the basis ofspectrometric data, primarily one-dimensional (1D)- and two-dimensional (2D)-NMR and massspectrometry. Electronic circular dichroism (ECD) calculations determined the absoluteconfiguration of . In addition, the isolated compounds were evaluated for their cytotoxic activityagainst three human tumor cell lines (HepG2, MCF-7, and HeLa). 6,12-Dihydroxyabieta-5,8,11,13-tetraen-7-one () showed moderate cytotoxicity against all three cell lines with IC50 values rangingfrom 24.41 μM to 58.39 μM and trilobinone (10) showed weaker activity with IC50 values rangingfrom 56.93 μM to 79.98 μM. None of the isolated diterpenoids have been previously reported from, and five compounds are here reported from the genus for the first time.
对植物全株的植物化学研究导致分离出一种以前未描述的拉伯烷二萜，（4,5,9,10）-13-去乙基-13-氧代拉伯烷-8(17),11-二烯-19-酸（），以及九种已知的二萜（-, -）、两种木脂素（, ）和一种香豆素（）。所有化合物的结构均基于光谱数据，主要是一维（1D）和二维（2D）NMR 和质谱确定。电子圆二色性（ECD）计算确定了 的绝对构型。此外，还评估了分离得到的化合物对三种人肿瘤细胞系（HepG2、MCF-7 和 HeLa）的细胞毒性。6,12-二羟基扁柏-5,8,11,13-四烯-7-酮（）对所有三种细胞系均显示出中等的细胞毒性，IC50 值范围为 24.41 μM 至 58.39 μM，而三螺诺酮（10）的活性较弱，IC50 值范围为 56.93 μM 至 79.98 μM。从没有以前报道过植物中分离出的二萜，并且有五种化合物是首次从属中分离得到的。
