Al-Qahtani Wahidah H, Binobead Manal Abdulaziz
College of Food & Agriculture Sciences, Department of Food Sciences & Nutrition, King Saud University, Riyadh, Saudi Arabia.
Saudi J Biol Sci. 2019 May;26(4):647-652. doi: 10.1016/j.sjbs.2018.01.003. Epub 2018 Feb 6.
has been advocated as safe food for human use by several investigators. In this study its beneficial dietary effect against liver injuries caused by d-galactosamine (d-GalN) was studied ensuring safety to human health using animal model. Acute hepatotoxicity was induced in Wister rats with d-GalN followed by treatment with butylated hydroxytoluene (BHT) and with Spirulina aqueous extract at various concentrations. The effect of Spirulina at different concentrations were tried and compared with BHT treatment. The animals treated with d-GalN on subsequent treatment by supplementation with Spirulina (6, 9%) in the diets, led to significant reversal in the levels of the antioxidant enzymes through hepatocytes by suppression of negative effect. Spirulina aqueous extract at 9% resulted in a significant decrease in the levels of alkaline phosphatase and infalmatory markers TNFα, IL6 and IL1β and also decreased TBARS, while it showed an increase in oxidative stress marker such as GR, GSH, GST, SOD, GPX and CAT and total protein when compared to the levels recorded with that group treated with d-GalN. Results also indicated that Spirulina aqueous extract at 9% concentration was equally effective in protecting liver damage as it was observed with BHT. Histological studies on liver treated with d-GalN, BHT and Spirulina aqueous extract showed that is effective as diet in providing beneficial protective effect. The results obtained in the present study very clearly indicated the positive beneficial protective effect of Spirulina, when used as diet, on the safety and protection of liver from injuries caused by toxicants.
几位研究人员主张将其作为供人类食用的安全食品。在本研究中，使用动物模型研究了其对由d-半乳糖胺（d-GalN）引起的肝损伤的有益饮食作用，同时确保对人类健康的安全性。用d-GalN诱导Wister大鼠急性肝毒性，随后用不同浓度的丁基羟基甲苯（BHT）和螺旋藻水提取物进行处理。尝试了不同浓度螺旋藻的效果，并与BHT处理进行比较。在用d-GalN处理的动物随后通过在饮食中补充螺旋藻（6%，9%）进行治疗后，通过抑制负面影响，导致抗氧化酶水平在肝细胞中显著逆转。9%的螺旋藻水提取物导致碱性磷酸酶水平以及炎症标志物TNFα、IL6和IL1β显著降低，同时TBARS也降低，而与用d-GalN处理的组记录的水平相比，它显示出氧化应激标志物如GR、GSH、GST、SOD、GPX和CAT以及总蛋白增加。结果还表明，9%浓度的螺旋藻水提取物在保护肝脏损伤方面与BHT同样有效。对用d-GalN、BHT和螺旋藻水提取物处理的肝脏进行的组织学研究表明，其作为饮食在提供有益的保护作用方面是有效的。本研究中获得的结果非常清楚地表明，螺旋藻作为饮食对肝脏免受毒物损伤的安全性和保护具有积极的有益保护作用。
