Mohammadi Mohsen, Shahisaraee Sabrieh Assadi, Tavajjohi Atiyeh, Pournoori Negin, Muhammadnejad Samad, Mohammadi Shahla Roodbar, Poursalehi Reza, Delavari H Hamid
Department of Materials Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, Iran.
Department of Medical Mycology, Tarbiat Modares University, Tehran, Iran.
IET Nanobiotechnol. 2019 Apr;13(2):114-119. doi: 10.1049/iet-nbt.2018.5146.
Fluconazole (FLZ) application as a highly successful commercial antifungal azole agent to treat the fungal infections is limited due to emergence of FLZ-resistant candida. In this study, the potential of green synthesised silver nanoparticles (NPs) as an antifungal agent against fungal pathogen is investigated. The extract of ginger () and thyme () plays as reducing agent, capping agent and antifungal agent. The UV-visible spectroscopy shows the peak of surface plasmon resonance of synthesised Ag NPs after a period of time. The synthesised Ag NPs are spherical, with average sizes of 12 and 18 nm based on ginger and thyme extract, respectively. Fourier transform infrared spectroscopy confirms the adsorption of the plant extract on the surface of the as-prepared Ag NPs. Based on the minimum inhibitory concentration (MIC) method against Candida albicans, the antifungal activity of as-prepared green synthesised Ag NPs shows higher inhibitory in comparison to FLZ. Finally, the Ag NPs synthesised via thyme extract shows no cytotoxicity with concentration below 3.5 ppm, which can be considered as an appropriate candidate instead of FLZ to treat the superficial fungal infections.
氟康唑（FLZ）作为一种非常成功的商业抗真菌唑类药物，由于耐氟康唑念珠菌的出现，其治疗真菌感染的应用受到限制。在本研究中，研究了绿色合成银纳米颗粒（NPs）作为抗真菌剂对抗真菌病原体的潜力。生姜（）和百里香（）的提取物充当还原剂、封端剂和抗真菌剂。紫外可见光谱显示一段时间后合成的银纳米颗粒的表面等离子体共振峰。合成的银纳米颗粒呈球形，基于生姜和百里香提取物，平均尺寸分别为12和18 nm。傅里叶变换红外光谱证实了植物提取物在制备的银纳米颗粒表面的吸附。基于针对白色念珠菌的最低抑菌浓度（MIC）方法，制备的绿色合成银纳米颗粒的抗真菌活性与氟康唑相比具有更高的抑制作用。最后，通过百里香提取物合成的银纳米颗粒在浓度低于3.5 ppm时无细胞毒性，可被视为替代氟康唑治疗浅表真菌感染的合适候选物。
