Tang Biao, Yu Yucong, Liang Junheng, Zhang Youming, Bian Xiaoying, Zhi Xiaoyang, Ding Xiaoming
Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
Collaborative Innovation Center for Genetics and Development, State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai 200438, PR China.
Int J Syst Evol Microbiol. 2019 Sep;69(9):2877-2883. doi: 10.1099/ijsem.0.003571.
Strain DSM 7029, isolated from a soil sample in Greece, can produce antitumour glidobactins, and has been found, as a heterologous host, to produce useful nonribosomal peptide synthetase-polyketide synthase hybrid molecules known as epothilones. This strain was originally named '' of the family and the order . However, phylogenetic analysis of the 16S rRNA gene sequence of strain DSM 7029 indicated that it was clustered with members of . Significant growth occurred at 25-42 °C, pH 5.0-10.0 and in the presence of 0-0.2 % (w/v) NaCl. The predominant ubiquinone was Q-8. The major fatty acids were Cω7/Cω6, C and Cω7. The G+C content of genomic DNA was 67.51 mol%. The strain was clearly distinguishable from other neighbouring members and genera and , using phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics and genome analysis. Therefore, strain DSM 7029 represents a novel species of the genus , for which the name sp. nov. is proposed.
从希腊土壤样本中分离出的DSM 7029菌株能够产生抗肿瘤的格利多菌素，并且作为异源宿主，已被发现能产生名为埃坡霉素的有用的非核糖体肽合成酶-聚酮合酶杂合分子。该菌株最初被命名为“”，属于“”科和“”目。然而，对DSM 7029菌株16S rRNA基因序列的系统发育分析表明，它与“”的成员聚类在一起。在25-42°C、pH 5.0-10.0以及存在0-0.2%（w/v）NaCl的条件下，该菌株能显著生长。主要泛醌为Q-8。主要脂肪酸为Cω7/Cω6、C和Cω7。基因组DNA的G+C含量为67.51 mol%。利用系统发育分析、脂肪酸组成数据以及一系列生理生化特征和基因组分析，该菌株与其他邻近的“”成员以及“”属和“”属明显区分开来。因此，DSM 7029菌株代表了“”属的一个新物种，为此提出新名称“” sp. nov.。
