Auer I O, Götz S, Ziemer E, Malchow H, Ehms H
Gut. 1979 Apr;20(4):261-8. doi: 10.1136/gut.20.4.261.
In the peripheral blood of patients with Crohn's disease (CD) the numerical distribution of the three major B lymphocyte subsets was determined by the identification of surface immunoglobulins using F(ab)(2)-antibody fragments. T cell counts were also obtained and the number of null cells was calculated. Twenty-eight patients with Crohn's disease including 14 patients with previously untreated and very short-standing disease (group CD 1) and 14 patients with long-standing and/or previous drug treated disease (group CD 2) were compared with 28 sex and age-matched normals as well as with 13 patients with acute inflammatory bowel disease (group D). Patients in group D and inactive patients of group CD 1 showed a significant absolute lymphocytosis due to an increase in both the three B cell subsets and the T cells, without changes in the null cells. While the proportion of T cells was normal, there was a significant relative B lymphocytosis and a relative null cytopenia in these patients. Active CD 1 patients, however, showed significantly lower absolute lymphocyte and T cell numbers. In group CD 2, there was a significant absolute lymphopenia caused by an equal decrease in B and T cells. Highly active CD 2 patients showed higher absolute null cell counts than inactive patients. With increasing disease duration there was a significant decrease of the relative and absolute B cell concentrations. The data obtained suggest that T and B cell populations in the peripheral blood are reduced in certain patients with Crohn's disease and that this occurs secondarily to activity of disease, chronicity of disease, and the effects of therapy.
采用F(ab)(2)抗体片段鉴定表面免疫球蛋白，测定克罗恩病（CD）患者外周血中三大主要B淋巴细胞亚群的数值分布。同时获取T细胞计数并计算裸细胞数量。将28例克罗恩病患者（包括14例未经治疗且病程极短的患者（CD 1组）和14例病程较长和/或曾接受药物治疗的患者（CD 2组））与28例年龄和性别匹配的正常人以及13例急性炎症性肠病患者（D组）进行比较。D组患者和CD 1组的非活动期患者出现显著的绝对淋巴细胞增多，这是由于三个B细胞亚群和T细胞均增加，而裸细胞无变化。虽然这些患者的T细胞比例正常，但存在显著的相对B淋巴细胞增多和相对裸细胞减少。然而，CD 1组的活动期患者绝对淋巴细胞和T细胞数量显著降低。在CD 2组，B细胞和T细胞同等程度减少导致显著的绝对淋巴细胞减少。CD 2组的高度活动期患者比非活动期患者的绝对裸细胞计数更高。随着病程延长，相对和绝对B细胞浓度显著降低。所获得的数据表明，某些克罗恩病患者外周血中的T细胞和B细胞群体减少，且这种情况继发于疾病活动、疾病慢性化及治疗效果。
