Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
Nanomedicine (Lond). 2019 Jul;14(13):1759-1769. doi: 10.2217/nnm-2019-0105. Epub 2019 Jul 12.
Rapid and recent progress in fluorescence microscopic techniques has allowed for routine discovery and viewing of biological structures and processes in unprecedented spatiotemporal resolution. In these imaging techniques, fluorescent nanoparticles (NPs) play important roles in the improvement of reporting systems. A short overview of recently developed fluorescent NPs used for advanced imaging will be discussed in this mini-review. The discussion begins with the contribution of fluorescence imaging in exploring the fate of NPs in biological systems. NP applications for imaging, including cell labeling, multimodal imaging and theranostic agents, are then discussed. Finally, despite all of the advancements in bioimaging, some unsolved challenges will be briefly discussed concerning future research directions.
荧光显微镜技术的快速发展和近期进展使人们能够以前所未有的时空分辨率常规发现和观察生物结构和过程。在这些成像技术中，荧光纳米粒子（NPs）在改进报告系统方面发挥着重要作用。本综述简要讨论了最近开发的用于高级成像的荧光 NPs。讨论首先从荧光成像在探索 NPs 在生物系统中命运方面的贡献开始。然后讨论了 NP 在成像中的应用，包括细胞标记、多模式成像和治疗诊断试剂。最后，尽管生物成像取得了所有进展，但针对未来研究方向，将简要讨论一些尚未解决的挑战。
