anti-inflammatory activity of L. bark using albumin denaturation method.

INTRODUCTION

In Ayurveda, many natural plant compounds are used to inhibit inflammatory pathways for centuries with less side effects. Different parts of L. () plant are used in Ayurveda for many diseases. However, few studies have been conducted to evaluate pharmacological activities of .

OBJECTIVE

The objective of the study was to analyze anti-inflammatory property of bark using albumin denaturation activity.

METHODOLOGY

bark extraction was performed using cold water and hot water. The concentration gradient of extracts was prepared using egg albumin and phosphate-buffered saline. The extract was incubated in a water bath at 37°C for 15 min and was heated at 70°C for 5 min. One nonsteroidal anti-inflammatory drug and one steroid were used as reference drugs. The percentage inhibition of protein denaturation was calculated.

RESULTS

The inhibition rate of egg albumin denaturation for water extraction increased gradually with concentration. Significantly higher inhibition was showed in hot water extracts than cold water extracts at the concentration of 0.01 μg/ml and 0.1 μg/ml. In addition, the inhibition rate of water extraction was significantly higher than the reference drugs ( < 0.05).

CONCLUSION

Anti-inflammatory activity increases with the concentration of bark. Furthermore, the action of this plant is significantly higher than the reference drugs.