Watanabe R A, Fryer J L, Rohovec J S
Department of Microbiology, Oregon State University, Corvallis 97331-3804.
Appl Environ Microbiol. 1988 Jun;54(6):1606-9. doi: 10.1128/aem.54.6.1606-1609.1988.
The effectiveness of tangential flow filtration (TFF) for the recovery of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) from large volumes of water was evaluated. In laboratory studies, virus recovery from IHNV-seeded water following concentration by TFF was approximately 13%. However, the addition of 0.1 and 1% fetal bovine serum to deionized water stabilized the virus, increasing virus recoveries to 95%. The addition of 0.03 and 0.3% beef extract resulted in IHNV recoveries of 80 and 61%, respectively. Similar results were obtained with IPNV-seeded water. Field studies using the TFF procedure were conducted with water from areas where IHNV is endemic. IHNV was detected in effluent from an adult steelhead trout (Salmo gairdneri) holding pond at an estimated concentration of 1 PFU/5 ml of water. It was also detected at levels of 1 PFU/50 ml in water from a 2-m-diameter circular tank containing IHNV-infected steelhead trout fry. IHNV isolated in samples taken from the Metolius River was detected by TFF at estimated levels of 1 PFU/3 liters.
评估了切向流过滤（TFF）从大量水中回收传染性造血坏死病毒（IHNV）和传染性胰腺坏死病毒（IPNV）的有效性。在实验室研究中，通过TFF浓缩后从接种了IHNV的水中回收病毒的效率约为13%。然而，向去离子水中添加0.1%和1%的胎牛血清可使病毒稳定，将病毒回收率提高到95%。添加0.03%和0.3%的牛肉浸出液后，IHNV的回收率分别为80%和61%。对接种了IPNV的水也得到了类似结果。使用TFF程序在IHNV流行地区的水域进行了现场研究。在一个成年虹鳟鱼（Salmo gairdneri）养殖池塘的流出水中检测到了IHNV，估计浓度为每5毫升水1个空斑形成单位（PFU）。在一个直径2米、装有感染IHNV的虹鳟鱼苗的圆形水箱的水中也检测到了每50毫升水1个PFU的IHNV。通过TFF在从梅托利厄斯河采集的样本中检测到的IHNV估计水平为每3升水1个PFU。
