Department of Oriental Pharmaceutical Science, College of Pharmacy and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, Dongdaemun-gu, Seoul, Republic of Korea.
Department of Medical Science of Meridian, Graduate School, Kyung Hee University, Dongdaemun-gu, Seoul, Republic of Korea.
PLoS One. 2019 Aug 12;14(8):e0217194. doi: 10.1371/journal.pone.0217194. eCollection 2019.
Recently, there has been a rapid increase in studies on the relationship between brain diseases and gut microbiota, and clinical evidence on gut microbial changes in Parkinson's disease (PD) has accumulated. 6-Hydroxydopamine (6-OHDA) is a widely used neurotoxin that leads to PD pathogenesis, but whether 6-OHDA affects gut microbial environment has not been investigated. Here we performed the 16S rRNA gene sequencing to analyze the gut microbial community of mice. We found that there were no significant changes in species richness and its diversity in the 6-OHDA-lesioned mice. The relative abundance of Lactobacillus gasseri and L. reuteri probiotic species in feces of 6-OHDA-lesioned mice was significantly decreased compared with those of sham-operated mice, while the commensal bacterium Bacteroides acidifaciens in 6-OHDA-treated mice was remarkably higher than sham-operated mice. These results provide a baseline for understanding the microbial communities of 6-OHDA-induced PD model to investigate the role of gut microbiota in the pathogenesis of PD.
最近，关于脑部疾病与肠道微生物群的关系的研究迅速增加，帕金森病（PD）中肠道微生物变化的临床证据也在不断积累。6-羟多巴胺（6-OHDA）是一种广泛使用的神经毒素，可导致 PD 发病机制，但 6-OHDA 是否会影响肠道微生物环境尚未得到研究。在这里，我们进行了 16S rRNA 基因测序，以分析小鼠的肠道微生物群落。我们发现，6-OHDA 损伤的小鼠的物种丰富度及其多样性没有显著变化。与假手术组相比，6-OHDA 损伤组小鼠粪便中乳杆菌属和雷特氏菌属益生菌的相对丰度明显降低，而 6-OHDA 处理组小鼠的共生菌拟杆菌属的含量明显高于假手术组。这些结果为了解 6-OHDA 诱导的 PD 模型中的微生物群落提供了一个基线，以研究肠道微生物群在 PD 发病机制中的作用。
