Department of Medicine, University of Vermont College of Medicine, Burlington, Vermont, United States.
Translational Airways Group, School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia.
Sci Rep. 2019 Sep 20;9(1):13671. doi: 10.1038/s41598-019-50223-y.
Flavored e-cigarettes are preferred by the majority of users yet their potential toxicity is unknown. Therefore our aim was to determine the effect of selected flavored e-cigarettes, with or without nicotine, on allergic airways disease in mice. Balb/c mice were challenged with PBS or house dust mite (HDM) (Days 0, 7, 14-18) and exposed to room air or e-cigarette aerosol for 30 min twice daily, 6 days/week from Days 0-18 (n = 8-12/group). Mice were exposed to Room Air, vehicle control (50%VG/%50PG), Black Licorice, Kola, Banana Pudding or Cinnacide without or with 12 mg/mL nicotine. Mice were assessed at 72 hours after the final HDM challenge. Compared to mice challenged with HDM and exposed to Room Air, nicotine-free Cinnacide reduced airway inflammation (p = 0.045) and increased peripheral airway hyperresponsiveness (p = 0.02), nicotine-free Banana Pudding increased soluble lung collagen (p = 0.049), with a trend towards increased airway inflammation with nicotine-free Black Licorice exposure (p = 0.089). In contrast, all e-cigarettes containing nicotine suppressed airway inflammation (p < 0.001 for all) but did not alter airway hyperresponsiveness or airway remodeling. Flavored e-cigarettes without nicotine had significant but heterogeneous effects on features of allergic airways disease. This suggests that some flavored e-cigarettes may alter asthma pathophysiology even when used without nicotine.
调味电子烟受到大多数用户的青睐，但它们的潜在毒性尚不清楚。因此，我们的目的是确定含有或不含有尼古丁的选定调味电子烟对小鼠过敏性气道疾病的影响。Balb/c 小鼠用 PBS 或屋尘螨（HDM）（第 0、7、14-18 天）进行攻毒，并在第 0-18 天（n=8-12/组）期间每天两次暴露于室内空气或电子烟气溶胶中 30 分钟，每周 6 天。将小鼠暴露于室内空气、载剂对照（50%VG/%50PG）、黑甘草味、可乐味、香蕉布丁味或肉桂味电子烟，或不含或含 12mg/ml 尼古丁的肉桂味电子烟。在最后一次 HDM 攻毒后 72 小时评估小鼠。与用 HDM 攻毒并暴露于室内空气的小鼠相比，不含尼古丁的肉桂味电子烟降低了气道炎症（p=0.045）并增加了外周气道高反应性（p=0.02），不含尼古丁的香蕉布丁增加了可溶性肺胶原蛋白（p=0.049），而不含尼古丁的黑甘草味电子烟暴露则有增加气道炎症的趋势（p=0.089）。相比之下，所有含有尼古丁的电子烟均抑制气道炎症（所有 p<0.001），但不改变气道高反应性或气道重塑。不含尼古丁的调味电子烟对过敏性气道疾病的特征具有显著但异质的影响。这表明，即使在不使用尼古丁的情况下，一些调味电子烟也可能改变哮喘的病理生理学。
