Tao Zhi, Zhang Lei, Zhang Qiongqiong, Lv Tao, Chen Rui, Wang Lijun, Huang Zhenyu, Hu Long, Liao Qinping
School of Clinical Medicine, Tsinghua University, Beijing 100084, People's Republic of China.
Department of Obstetrics and Gynecology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing 102218, People's Republic of China.
Infect Drug Resist. 2019 Dec 4;12:3745-3754. doi: 10.2147/IDR.S227883. eCollection 2019.
Aerobic vaginitis (AV) is a newly defined type of bacterial vaginitis, but its pathogenesis is not yet clear. appears as an emerging pathogen in recent case reports, and colonizes in vagina of patients with AV. In this study, we investigate the pathogenesis of in AV.
(1) We collected 41 vaginal specimens from 21 healthy, fertile women with normal vaginal flora (NM), 10 with bacterial vaginosis (BV) and 10 with AV; their microbiome structure was analysed by 16S rRNA gene sequencing. (2) and vaginal epithelial cells were cocultured in vitro, and cytotoxicity was tested by an LDH kit. (3) The virulence gene sag was knocked out, and the cytotoxicity of the mutant in vaginal epithelial cells was tested.
(1) The microbiome structure of AV was dramatically different from that of BV and NM. The predominant genera of the three groups were . and . Streptococcus spp. were significantly more abundant in AV than in BV (95% CI [0.1391, 0.8676], P<0.01) and NM (95% CI [0.1391, 0.8676], P<0.01). (2) was the dominant species in AV (95% CI [0.04672, 0.1097], P<0.01). (3) The mean cytotoxicity of in vaginal epithelial cells was 58.34% for the wild type (WT) and 16.43% for the mutant; this difference was significant (95% CI [-60.55, -23.28], P<0.01).
was the predominant microorganism in patients with AV in our study. caused vaginal epithelial cell lysis, indicating that is an AV pathogen. The virulence gene sag is vital for vaginal epithelial cell lysis.
需氧菌性阴道炎（AV）是一种新定义的细菌性阴道炎类型，但其发病机制尚不清楚。在最近的病例报告中作为一种新兴病原体出现，并在AV患者的阴道中定植。在本研究中，我们调查了其在AV中的发病机制。
（1）我们收集了21名阴道菌群正常的健康育龄妇女（NM）、10名患有细菌性阴道病（BV）和10名患有AV的妇女的41份阴道标本；通过16S rRNA基因测序分析其微生物群结构。（2）与阴道上皮细胞进行体外共培养，并通过乳酸脱氢酶试剂盒检测细胞毒性。（3）敲除其毒力基因sag，并检测突变体在阴道上皮细胞中的细胞毒性。
（1）AV的微生物群结构与BV和NM的显著不同。三组的优势菌属分别是。和。链球菌属在AV中比在BV（95%可信区间[0.1391，0.8676]，P<0.01）和NM（95%可信区间[0.1391，0.8676]，P<0.01）中明显更丰富。（2）是AV中的优势菌种（95%可信区间[0.04672，0.1097]，P<0.01）。（3）野生型在阴道上皮细胞中的平均细胞毒性为58.34%，突变体为16.43%；这种差异具有统计学意义（95%可信区间[-60.55，-23.28]，P<0.01）。
在我们的研究中是AV患者中的主要微生物。导致阴道上皮细胞裂解，表明是一种AV病原体。毒力基因sag对阴道上皮细胞裂解至关重要。
