The Pathogenesis Of In Aerobic Vaginitis.

BACKGROUND

Aerobic vaginitis (AV) is a newly defined type of bacterial vaginitis, but its pathogenesis is not yet clear. appears as an emerging pathogen in recent case reports, and colonizes in vagina of patients with AV. In this study, we investigate the pathogenesis of in AV.

MATERIALS AND METHODS

(1) We collected 41 vaginal specimens from 21 healthy, fertile women with normal vaginal flora (NM), 10 with bacterial vaginosis (BV) and 10 with AV; their microbiome structure was analysed by 16S rRNA gene sequencing. (2) and vaginal epithelial cells were cocultured in vitro, and cytotoxicity was tested by an LDH kit. (3) The virulence gene sag was knocked out, and the cytotoxicity of the mutant in vaginal epithelial cells was tested.

RESULTS

(1) The microbiome structure of AV was dramatically different from that of BV and NM. The predominant genera of the three groups were . and . Streptococcus spp. were significantly more abundant in AV than in BV (95% CI [0.1391, 0.8676], P<0.01) and NM (95% CI [0.1391, 0.8676], P<0.01). (2) was the dominant species in AV (95% CI [0.04672, 0.1097], P<0.01). (3) The mean cytotoxicity of in vaginal epithelial cells was 58.34% for the wild type (WT) and 16.43% for the mutant; this difference was significant (95% CI [-60.55, -23.28], P<0.01).

CONCLUSION

was the predominant microorganism in patients with AV in our study. caused vaginal epithelial cell lysis, indicating that is an AV pathogen. The virulence gene sag is vital for vaginal epithelial cell lysis.