Department of Gynaecology and Obstetrics, Sanmenxia Central Hospital of Henan University of Science and Technology, Sanmenxia, 472000, Henan, China.
Geneis (Beijing) Co.Ltd, Beijing, 100102, China.
Sci Rep. 2019 Dec 18;9(1):19337. doi: 10.1038/s41598-019-55843-y.
Human papillomavirus (HPV) is a major pathogen that causes cervical cancer and many other related diseases. HPV infection related cervical microbiome could be an induce factor of cervical cancer. However, it is uncommon to find a single test on the market that can simultaneously provide information on both HPV and the microbiome. Herein, a novel method was developed in this study to simultaneously detect HPV infection and microbiota composition promptly and accurately. It provides a new and simple way to detect vaginal pathogen situation and also provide valuable information for clinical diagnose. This approach combined multiplex PCR, which targeted both HPV16 E6E7 and full-length 16S rRNA, and Nanopore sequencing to generate enough information to understand the vagina condition of patients. One HPV positive liquid-based cytology (LBC) sample was sequenced and analyzed. After comparing with Illumina sequencing, the results from Nanopore showed a similar microbiome composition. An instant sequencing evaluation showed that 15 min sequencing is enough to identify the top 10 most abundant bacteria. Moreover, two HPV integration sites were identified and verified by Sanger sequencing. This approach has many potential applications in pathogen detection and can potentially aid in providing a more rapid clinical diagnosis.
人乳头瘤病毒（HPV）是导致宫颈癌和许多其他相关疾病的主要病原体。HPV 感染相关的宫颈微生物组可能是宫颈癌的诱发因素。然而，市场上很少有一种单一的检测方法可以同时提供 HPV 和微生物组的信息。本研究开发了一种新的方法，可以快速、准确地同时检测 HPV 感染和微生物群落组成。它为检测阴道病原体情况提供了一种新的简单方法，并为临床诊断提供了有价值的信息。该方法结合了靶向 HPV16 E6E7 和全长 16S rRNA 的多重 PCR 和纳米孔测序，以生成足够的信息来了解患者的阴道状况。对一个 HPV 阳性的液基细胞学（LBC）样本进行了测序和分析。与 Illumina 测序结果比较后，纳米孔测序结果显示出相似的微生物群落组成。即时测序评估表明，15 分钟的测序足以识别前 10 种最丰富的细菌。此外，通过 Sanger 测序鉴定和验证了两个 HPV 整合位点。这种方法在病原体检测中有许多潜在的应用，有可能有助于提供更快速的临床诊断。
