The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, 2800, Denmark.
Department of Applied Mathematics and Computer Science, Technical University of Denmark, Kgs. Lyngby, 2800, Denmark.
Anal Chem. 2020 Mar 17;92(6):4317-4325. doi: 10.1021/acs.analchem.9b05060. Epub 2020 Feb 11.
Reusability of sensors is relevant when aiming to decrease variation between measurements, as well as cost and time of analysis. We present an electrochemically assisted surface-enhanced Raman spectroscopy (SERS) platform with the capability to reverse the analyte-surface interaction, without damaging the SERS substrate, allowing for efficient sensor reuse. The platform was used in combination with a sample pretreatment step, when detecting melamine from milk. We found that the electrochemically enhanced analyte-surface interaction results in significant improvement in detection sensitivity, with detection limits (0.01 ppm in PBS and 0.3 ppm in milk) below the maximum allowed levels in food samples. The reversibility of interaction enabled continuous measurement in aqueous solution and a complete quantitative assay on a single SERS substrate.
当旨在减少测量之间的差异、降低分析成本和时间时，传感器的可重复使用性就显得尤为重要。我们提出了一种电化学辅助的表面增强拉曼光谱（SERS）平台，该平台具有在不损坏 SERS 基底的情况下逆转分析物-表面相互作用的能力，从而实现传感器的高效重复使用。该平台与样品预处理步骤结合使用，用于检测牛奶中的三聚氰胺。我们发现，电化学增强的分析物-表面相互作用显著提高了检测灵敏度，检测限（PBS 中为 0.01ppm，牛奶中为 0.3ppm）低于食品样品中允许的最高水平。相互作用的可逆性使得在水溶液中能够进行连续测量，并在单个 SERS 基底上完成完全定量分析。
