LAMA - 1：一种从深海真菌中分离出的脑苷脂。

LAMA-1: A Cerebroside Isolated from the Deep-Sea-Derived Fungus .

作者信息

Alshehri Samah O, Malatani Rania T, Bogari Hanin A, Noor Ahmad O, Ibrahim Amany K, Elhady Sameh S, Abdelhameed Reda F A

机构信息

Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.

出版信息

Metabolites. 2020 Feb 20;10(2):75. doi: 10.3390/metabo10020075.

DOI:10.3390/metabo10020075

PMID:32093136

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7074484/

Abstract

Chemical investigation of the ethyl acetate extract of strain S003, a fungus isolated from Red Sea deep sediment, led to the isolation of a cerebroside molecular species LAMA () along with three other known compounds, ergosterol (), epidioxyergosterol (), and kojic acid (). The structures of the isolated compounds were elucidated by interpretation of spectral data, including detailed 1D and 2D NMR (One and two dimensional Nuclear Magnetic Resonance) and mass spectrometry. The cytotoxic activities of isolated compounds - against five human carcinoma cells were evaluated using sulforhodamine B (SRB) assay. Compounds and displayed promising cytotoxic profiles against lung cancer (A-549), prostate (DU-145), breast adenocarcinoma (MCF-7), and hepatocellular (HepG2) cell lines, with IC values of 21.26, 19.3; 1.50, 6.10; 16.95, 13.6; and 2.89, 3.07 µM, respectively, while they were inactive against HeLa cells. Compounds and showed weak cytotoxic profiles against all cell lines under investigation.

摘要

对从红海深海沉积物中分离出的菌株S003的乙酸乙酯提取物进行化学研究，分离出一种脑苷脂分子物种LAMA（）以及其他三种已知化合物，即麦角固醇（）、表氧化麦角固醇（）和曲酸（）。通过对光谱数据的解析，包括详细的一维和二维核磁共振（1D和2D NMR）以及质谱分析，阐明了分离出的化合物的结构。使用磺酰罗丹明B（SRB）测定法评估了分离出的化合物对五种人类癌细胞的细胞毒性活性。化合物和对肺癌（A - 549）、前列腺癌（DU - 145）、乳腺腺癌（MCF - 7）和肝癌（HepG2）细胞系显示出有前景的细胞毒性谱，IC值分别为21.26、19.3；1.50、6.10；16.95、13.6；和2.89、3.07 μM，而它们对HeLa细胞无活性。化合物和对所有研究的细胞系显示出较弱的细胞毒性谱。