Smailagić Anita, Ristivojević Petar, Dimkić Ivica, Pavlović Tamara, Dabić Zagorac Dragana, Veljović Sonja, Fotirić Akšić Milica, Meland Mekjell, Natić Maja
Innovation Center of the Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia.
Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia.
Foods. 2020 Mar 10;9(3):319. doi: 10.3390/foods9030319.
The main focus of this study is to assess radical scavenging and antimicrobial activities of the 11 wood extracts: oak ( (Matt.) Liebl. L., and L.), mulberry ( L.), myrobalan plum ( Ehrh.), black locust ( L.), and wild cherry ( L.). High-performance thin-layer chromatography (HPTLC) provided initial phenolic screening and revealed different chemical patterns among investigated wood extracts. To identify individual compounds with radical scavenging activity DPPH-HPTLC, assay was applied. Gallic acid, ferulic and/or caffeic acids were identified as the compounds with the highest contribution of total radical scavenging activity. Principal component analysis was applied on the data set obtained from HPTLC chromatogram to classify samples based on chemical fingerprints: spp. formed separate clusters from the other wood samples. The wood extracts were evaluated for their antimicrobial activity against eight representative human and opportunistic pathogens. The lowest minimum inhibitory concentration (MIC) was recorded against for black locust, cherry and mulberry wood extracts. This work provided simple, low-cost and high-throughput screening of phenolic compounds and assessments of the radical scavenging properties of selected individual metabolites from natural matrix that contributed to scavenge free radicals.
本研究的主要重点是评估11种木材提取物的自由基清除和抗菌活性：橡木（（马特。）利布尔。L.和L.）、桑树（L.）、诃子（Ehrh.）、刺槐（L.）和野樱桃（L.）。高效薄层色谱法（HPTLC）进行了初步的酚类筛选，并揭示了所研究木材提取物之间不同的化学模式。为了鉴定具有自由基清除活性的单个化合物，应用了DPPH-HPTLC测定法。没食子酸、阿魏酸和/或咖啡酸被鉴定为对总自由基清除活性贡献最大的化合物。对从HPTLC色谱图获得的数据集进行主成分分析，以根据化学指纹对样品进行分类：spp.与其他木材样品形成单独的簇。评估了木材提取物对八种代表性人类和机会性病原体的抗菌活性。刺槐、樱桃和桑树木材提取物对的最低抑菌浓度（MIC）记录在案。这项工作提供了简单、低成本和高通量的酚类化合物筛选，以及对来自天然基质的选定单个代谢物的自由基清除特性的评估，这些代谢物有助于清除自由基。
