在活细胞、组织和动物中进行基因靶向的化学组装功能材料。
Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals.
机构信息
Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
出版信息
Science. 2020 Mar 20;367(6484):1372-1376. doi: 10.1126/science.aay4866.
The structural and functional complexity of multicellular biological systems, such as the brain, are beyond the reach of human design or assembly capabilities. Cells in living organisms may be recruited to construct synthetic materials or structures if treated as anatomically defined compartments for specific chemistry, harnessing biology for the assembly of complex functional structures. By integrating engineered-enzyme targeting and polymer chemistry, we genetically instructed specific living neurons to guide chemical synthesis of electrically functional (conductive or insulating) polymers at the plasma membrane. Electrophysiological and behavioral analyses confirmed that rationally designed, genetically targeted assembly of functional polymers not only preserved neuronal viability but also achieved remodeling of membrane properties and modulated cell type-specific behaviors in freely moving animals. This approach may enable the creation of diverse, complex, and functional structures and materials within living systems.
多细胞生物系统（如大脑）的结构和功能复杂性超出了人类设计或组装能力的范围。如果将生物体中的细胞视为具有特定化学性质的解剖定义隔室进行处理，那么它们可以被招募来构建合成材料或结构，从而利用生物学来组装复杂的功能结构。通过整合工程酶靶向和聚合物化学，我们通过基因指令使特定的活神经元引导等离子体膜中电功能（导电或绝缘）聚合物的化学合成。电生理和行为分析证实，经过合理设计、基因靶向组装的功能聚合物不仅能保持神经元活力，还能重塑膜特性，并调节自由活动动物中特定细胞类型的行为。这种方法可能使我们能够在活的生物系统中创建多样化、复杂和功能化的结构和材料。
Zotero 插件