Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, Cidade Universitária, Campinas, SP, 13083- 862, Brazil.
Biotechnol Lett. 2020 Jun;42(6):875-884. doi: 10.1007/s10529-020-02875-4. Epub 2020 Apr 1.
Second-generation bioethanol is a sustainable energy source that can be produced from different renewable materials. However, there is a challenge we must overcome to significantly enhance bioethanol production: the hydrolysis of lignocellulosic biomass to fermentable sugars. Synergistic enzymes, such as endoglucanases, β-glucosidases, cellobiohydrolases, and, more recently, lytic polysaccharide monooxygenases and cellobiose dehydrogenases have been used with great success to hydrolyze pretreated biomass. Further advances in the field of second-generation bioethanol production will likely depend on an increased understanding of the interactions between enzymes and lignocellulosic substrates, the development of enzyme engineering, and the optimization of enzyme mixtures to enhance cellulose hydrolysis.
第二代生物乙醇是一种可持续的能源，可以从不同的可再生材料中生产。然而，要显著提高生物乙醇的产量，我们必须克服一个挑战：将木质纤维素生物质水解为可发酵糖。协同酶，如内切葡聚糖酶、β-葡萄糖苷酶、纤维二糖水解酶，以及最近的溶菌多糖单加氧酶和纤维二糖脱氢酶，已经被成功地用于水解预处理的生物质。第二代生物乙醇生产领域的进一步进展可能取决于对酶与木质纤维素底物之间相互作用的深入了解、酶工程的发展以及酶混合物的优化，以提高纤维素水解。
