Asmerom Demoze, Hailu Gebremedhin Solomon, Yimer Ebrahim M, Bitew Helen, Kahsay Getu
Department of Medicinal Chemistry, School of Pharmacy, College of Health Sciences, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia.
Department of Pharmacology, School of Pharmacy, College of Health Sciences, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia.
Evid Based Complement Alternat Med. 2020 Mar 27;2020:8312471. doi: 10.1155/2020/8312471. eCollection 2020.
The highest prevalence and emergence of microbial infections coupled with the threat of antimicrobial resistance constitute a global concern, which entails searching for novel antimicrobial agents. Medicinal plants are among the major sources of medicines for novel drug discovery. is one of the endemic species in Ethiopia where the leaf latex of the plant is traditionally used for the treatment of various pathogenic conditions such as wound, dandruff, malaria, and diabetes. In spite of such claims, there was no scientific study done so far. The aim of the current study was, therefore, to evaluate the antimicrobial effect of leaf latex of and its thin layer chromatography (TLC) fractions.
Thin layer chromatography (TLC) separation was employed for isolation of bioactive compounds. Agar well diffusion and microdilution assay method were used to evaluate the antimicrobial actions of the leaf latex and TLC fractions against six bacterial strains and four species of reference and clinical isolate microbial strains.
Three major fractions, AA01, AA02, and AA03, were identified by TLC. Among the tested microbial strains, the reference strain of ATCC 29213 (MIC = 0.06 mg/mL) and clinical 242/18 (MIC = 0.14 mg/mL) exhibited higher susceptibility towards AA02, while reference strains of ATCC 700603 (MIC = 0.19 mg/mL) revealed the highest susceptibility towards AA01. The leaf latex displayed the highest activity against ATCC 29213 and clinical 242/18 with a MIC value of 0.19 mg/mL.
The leaf latex and TLC fractions were found to be active against the tested bacterial and species. Therefore, this finding supports the traditional claim of and the need for characterization of the TLC fractions to provide as lead compounds for further comprehensive antibacterial and antifungal activities.
微生物感染的高患病率和新出现的感染，再加上抗菌药物耐药性的威胁，构成了一个全球关注的问题，这就需要寻找新型抗菌剂。药用植物是新药发现的主要药物来源之一。[植物名称]是埃塞俄比亚的特有物种之一，该植物的叶乳胶传统上用于治疗各种致病病症，如伤口、头皮屑、疟疾和糖尿病。尽管有这些说法，但迄今为止尚未进行科学研究。因此，本研究的目的是评估[植物名称]叶乳胶及其薄层色谱（TLC）馏分的抗菌效果。
采用薄层色谱（TLC）分离法分离生物活性化合物。采用琼脂孔扩散法和微量稀释法评估叶乳胶和TLC馏分对六种细菌菌株以及四种参考和临床分离微生物菌株的抗菌作用。
通过TLC鉴定出三个主要馏分，即AA01、AA02和AA03。在测试的微生物菌株中，[细菌名称]ATCC 29213参考菌株（MIC = 0.06 mg/mL）和临床[细菌名称]242/18（MIC = 0.14 mg/mL）对AA02表现出较高的敏感性，而[细菌名称]ATCC 700603参考菌株（MIC = 0.19 mg/mL）对AA01表现出最高的敏感性。叶乳胶对[细菌名称]ATCC 292
