Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil.
School of Dental Medicine, University of Connecticut (UCONN), Farmington, CT, USA.
Biofouling. 2020 Apr;36(4):428-441. doi: 10.1080/08927014.2020.1769613. Epub 2020 May 26.
Titanium-zirconium (Ti-Zr) alloy has been widely used as a biomaterial for implant devices, and it is commonly treated by sandblasting followed by acid etching (SLA) to improve biological responses. Although protein adsorption is the first biological response, the effect of this SLA treatment on the proteomic profile of proteins adsorbed from saliva and blood plasma has not been tested. In this study, the proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). was used to test whether the protein layer affects bacterial adhesion. SLA treatment affected the proteomic profile, showing exclusive proteins adsorbed from saliva (14) and plasma (3). However, both groups exhibited close patterns of intensity for common proteins, molecular functions and biological processes mediated by proteins. Interestingly, Ti-Zr showed higher bacterial adhesion (∼1.9 fold over) for the surface coated with plasma proteins. Therefore, SLA treatment of Ti-Zr alloy changed the proteomic profile, which may affect bacterial adhesion.
钛锆（Ti-Zr）合金已被广泛用作植入设备的生物材料，通常通过喷砂后酸蚀（SLA）处理来改善生物响应。虽然蛋白质吸附是第一个生物响应，但这种 SLA 处理对从唾液和血浆中吸附的蛋白质的蛋白质组学图谱的影响尚未得到测试。在这项研究中，通过液相色谱与串联质谱（LC-MS/MS）评估了蛋白质组学图谱。使用来测试蛋白质层是否影响细菌粘附。SLA 处理影响了蛋白质组学图谱，显示出从唾液（14）和血浆（3）中吸附的独特蛋白质。然而，两组对于常见蛋白质的强度模式都很接近，这些蛋白质由分子功能和生物过程介导。有趣的是，Ti-Zr 对涂覆有血浆蛋白的表面表现出更高的细菌粘附（约 1.9 倍）。因此，Ti-Zr 合金的 SLA 处理改变了蛋白质组学图谱，这可能影响细菌粘附。
