Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Department of Development and Stem Cells, CNRS (UMR 7104), Inserm U964, Université de Strasbourg, 67404, Illkirch Graffenstaden, France.
Department of Pathology, Brigham and Women's Hospital, 60 Fenwood Road, Boston, MA 02115, USA.
Development. 2020 Jun 26;147(12):dev187344. doi: 10.1242/dev.187344.
Satellite cells (SC) are muscle stem cells that can regenerate adult muscles upon injury. Most SC originate from PAX7 myogenic precursors set aside during development. Although myogenesis has been studied in mouse and chicken embryos, little is known about human muscle development. Here, we report the generation of human induced pluripotent stem cell (iPSC) reporter lines in which fluorescent proteins have been introduced into the and loci. We use single cell RNA sequencing to analyze the developmental trajectory of the iPSC-derived PAX7 myogenic precursors. We show that the PAX7 cells generated in culture can produce myofibers and self-renew and Together, we demonstrate that cells exhibiting characteristics of human fetal satellite cells can be produced from iPSC, opening interesting avenues for muscular dystrophy cell therapy. This work provides significant insights into the development of the human myogenic lineage.
卫星细胞（SC）是肌肉干细胞，可在受伤后再生成人肌肉。大多数 SC 来源于发育过程中分离出来的 PAX7 成肌前体细胞。虽然在小鼠和鸡胚中已经研究了肌发生，但对人类肌肉发育知之甚少。在这里，我们报告了人类诱导多能干细胞（iPSC）报告系的产生，其中荧光蛋白已被引入 和 基因座。我们使用单细胞 RNA 测序来分析 iPSC 衍生的 PAX7 成肌前体细胞的发育轨迹。我们表明，在培养中产生的 PAX7 细胞可以产生肌纤维并自我更新 和 。总之，我们证明了可以从 iPSC 中产生具有人胎儿卫星细胞特征的细胞，为肌肉营养不良的细胞治疗开辟了有趣的途径。这项工作为人类成肌谱系的发育提供了重要的见解。
