循环外泌体长链非编码 RNA H19 作为胃癌潜在的新型诊断和预后生物标志物。
Circulating exosomal long non-coding RNA H19 as a potential novel diagnostic and prognostic biomarker for gastric cancer.
机构信息
Department of Clinical Laboratory, The Affiliated Hospital of Jiaxing University, Jiaxing, China.
出版信息
J Int Med Res. 2020 Jul;48(7):300060520934297. doi: 10.1177/0300060520934297.
OBJECTIVE
Long non-coding RNAs (lncRNAs) are involved in carcinogenesis and could be used as diagnostic biomarkers. Our study aimed to elucidate the clinical role of serum exosomal lncRNA H19 in gastric cancer (GC).
METHODS
In this prospective clinical study, we determined serum exosomal lncRNA H19 levels in 81 patients with GC and analysed the correlations between serum lncRNA H19 levels and clinical characteristics. Receiver operating characteristics (ROC) curves were constructed to determine the diagnostic performance of exosomal lncRNA H19 in GC.
RESULTS
Serum exosomal lncRNA H19 levels were significantly upregulated in patients with GC both before and after surgery compared with healthy controls. Furthermore, serum exosomal lncRNA H19 levels were significantly decreased after compared with before surgery in patients with GC. Preoperative lncRNA H19 levels were significantly correlated with TNM stage. The area under the ROC curve (AUC) value for exosomal lncRNA H19 was 0.849, which was significantly higher than the AUC values for cancer antigens 19-9 and 72-4 and carcinoembryonic antigen, either alone or combined.
CONCLUSIONS
These results suggest that circulating exosomal lncRNA H19 may be a potential biomarker with diagnostic and prognostic value in GC.
目的
长链非编码 RNA（lncRNA）参与癌症的发生，可作为诊断生物标志物。本研究旨在阐明血清外泌体 lncRNA H19 在胃癌（GC）中的临床作用。
方法
在这项前瞻性临床研究中，我们测定了 81 例 GC 患者的血清外泌体 lncRNA H19 水平，并分析了血清 lncRNA H19 水平与临床特征之间的相关性。绘制受试者工作特征（ROC）曲线以确定外泌体 lncRNA H19 在 GC 中的诊断性能。
结果
GC 患者术前和术后血清外泌体 lncRNA H19 水平均明显高于健康对照组。此外，GC 患者术后血清外泌体 lncRNA H19 水平明显低于术前。术前 lncRNA H19 水平与 TNM 分期显著相关。外泌体 lncRNA H19 的 ROC 曲线下面积（AUC）值为 0.849，明显高于单独或联合使用癌抗原 19-9、72-4 和癌胚抗原的 AUC 值。
结论
这些结果表明，循环外泌体 lncRNA H19 可能是 GC 具有诊断和预后价值的潜在生物标志物。
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