Cheng Huan, Xiao Dongdong, Tang Yujing, Wang Bijia, Feng Xueling, Lu Mujun, Vancso G Julius, Sui Xiaofeng
Key Lab of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China.
Materials Science and Technology of Polymers, MESA+ Institute of Nanotechnology, University of Twente, P.O. Box 217, Enschede, AE 7500, The Netherlands.
Adv Healthc Mater. 2020 Sep;9(17):e1901796. doi: 10.1002/adhm.201901796. Epub 2020 Jul 21.
The development of a rapid and effective hemostatic dressing is highly desired in the treatment of hemorrhagic wounds. In this study, sponges with Janus character are developed using cellulose nanofibers (CNFs) that exhibit materials facets of different wettability characteristics using heterogeneous mixing and freeze-drying. The bonding of the interface between the hydrophilic and hydrophobic facets is achieved by using interpenetrating chemical cross-linking between CNFs and organosilanes. The hydrophilic layer absorbs water from blood and works synergistically with the inherent hemostatic chitosan-rich complementary layer to accelerate blood clotting, displaying both active and passive hemostatic mechanisms. The hydrophobic layer prevents blood penetration into the construct and exerts proper pressure on the wound. Compared with the hydrophilic control samples and commercial gauzes, the Janus sponges can achieve effective bleeding control with nearly 50% less blood loss in a femoral artery injury model and prolong the survival time in a carotid artery injury model. Compared with the only hydrophilic layer, the time to hemostasis of Janus sponge are reduced from 165 ± 20 to 131 ± 26 s in femoral artery injury model and from 102 ± 21 to 83 ± 15 s in liver femoral artery injury model.
在出血性伤口的治疗中，迫切需要开发一种快速有效的止血敷料。在本研究中，利用纤维素纳米纤维（CNF）制备具有双面特性的海绵，通过非均相混合和冷冻干燥展现出具有不同润湿性特征的材料面。亲水性和疏水面之间的界面结合是通过CNF与有机硅烷之间的互穿化学交联实现的。亲水层从血液中吸收水分，并与富含壳聚糖的固有止血互补层协同作用以加速血液凝固，展现出主动和被动止血机制。疏水层可防止血液渗入结构体，并对伤口施加适当压力。与亲水性对照样品和商用纱布相比，双面海绵在股动脉损伤模型中可有效控制出血，失血量减少近50%，并在颈动脉损伤模型中延长存活时间。与仅具有亲水层相比，双面海绵在股动脉损伤模型中的止血时间从165±20秒减少到131±26秒，在肝股动脉损伤模型中从102±21秒减少到83±15秒。
